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Human Semaphorin 7A Antibody

Catalog #: AF2068
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2068
AF2068-SP
Detection of Semaphorin 7A in PBMC lymphocytes treated +/- 5 µg/mL PHA for 2 days by Flow Cytometry
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Product Details
Citations (5)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Semaphorin 7A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Semaphorin 7A in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Semaphorin 7A is observed. 
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 7A
His47-Ala648
Accession # O75326
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Semaphorin 7A
Flow Cytometry
0.25 µg/106 cells
Human T cells treated with PHA (see details below); HT1080 human fibrosarcoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Semaphorin 7A in PBMC lymphocytes treated +/- 5 µg/mL PHA for 2 days by Flow Cytometry PBMC lymphocytes treated with 5 µg/mL PHA for 2 days (A) vs naïve PBMC lymphocytes (B) were stained with Goat Anti-Human Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2068) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) and Mouse anti-Human CD3 APC-conjugated Monoclonal antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of Semaphorin 7A in HT1080 cells by Flow Cytometry HT1080 cells were stained with Goat Anti-Human Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2068, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 7A

Semaphorin 7A (Sema 7A), also known as Sema L, Sema K1 and CD108, is an 80 kDa glycosylphosphatidylinositol-anchored protein belonging to the Semaphorin superfamily. It binds to the beta 1 integrin subunit as well as Plexin C1. The Sema domain of human Sema 7A shares 92% and 90% amino acid sequence homology with that of the mouse and canine protein.

Entrez Gene IDs
8482 (Human); 20361 (Mouse)
Alternate Names
CD108 antigen; CD108; CD108MGC126696; CDw108; H-SEMA-K1; H-Sema-L; JMH blood group antigen; JMH; John-Milton-Hargen human blood group Ag; MGC126692; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A (JMH blood group); sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin)7A; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A; Sema K1; Sema L; Sema7A; SEMAK1; SEMAL; SEMALJohn Milton Hagen blood group H-Sema K1; Semaphorin 7A; semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group); semaphorin K1; semaphorin L; semaphorin-7A; semaphorin-K1; semaphorin-L

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Citations for Human Semaphorin 7A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Syngeneic mouse model of human HER2+ metastatic breast cancer for the evaluation of trastuzumab emtansine combined with oncolytic rhabdovirus
    Authors: Zaid Taha, Mathieu J.F. Crupi, Nouf Alluqmani, Faiha Fareez, Kristy Ng, Judy Sobh et al.
    Frontiers in Immunology
  2. Red blood cell-derived semaphorin 7A promotes thrombo-inflammation in myocardial ischemia-reperfusion injury through platelet GPIb
    Authors: D Köhler, T Granja, J Volz, M Koeppen, HF Langer, G Hansmann, E Legchenko, T Geisler, T Bakchoul, C Eggstein, HA Häberle, B Nieswandt, P Rosenberge
    Nat Commun, 2020-03-11;11(1):1315.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Endogenous Semaphorin-7A Impedes Human Lung Fibroblast Differentiation
    Authors: S Esnault, EE Torr, K Bernau, MW Johansson, EA Kelly, N Sandbo, NN Jarjour
    PLoS ONE, 2017-01-17;12(1):e0170207.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Semaphorin 7A Aggravates Pulmonary Inflammation during Lung Injury.
    Authors: Roth J, Kohler D, Schneider M, Granja T, Rosenberger P
    PLoS ONE, 2016-01-11;11(1):e0146930.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  5. IL-3 Maintains Activation of the p90S6K/RPS6 Pathway and Increases Translation in Human Eosinophils.
    Authors: Esnault S, Kelly E, Shen Z, Johansson M, Malter J, Jarjour N
    J Immunol, 2015-08-14;195(6):2529-39.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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