Human Serpin A11 Antibody

Catalog #: MAB8236 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8236
MAB8236-SP

Detection of Human Serpin A11 by Western Blot.

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All Serpin A11 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Serpin A11 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SerpinA11 in ELISA and Western Blot.

Source

Monoclonal Mouse IgG_2B Clone # 895842

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SerpinA11
Met1-Gly422
Accession # Q86U17

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Serpin A11 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin A11 Monoclonal Antibody (Catalog # MAB8236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin A11 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A11

Serpin A11 (serine proteinase inhibitor – clade A#11) is a 50-60 kDa secreted member of the serpin superfamily of protease inhibitors. Serpins are the largest family of endogenous protease inhibitors, and target a wide variety of serine and cysteine proteases. In general, members of this superfamily regulate multiple proteolytic cascades, and are particularly effective due to the fact that their inhibitory activities can be fine-tuned through the participation of discrete, non-serpin co-factors. Serpin A11 gene expression is extremely high in normal liver, with lower levels detected in normal breast, breast tumor, and benign prostatic hyperplasia. Human Serpin A11 is 422 amino acids (aa) in length, with four potential N-linked glycosylation sites, and shares 75% aa sequence identity with mouse Serpin A11.

Long Name

Serpin Peptidase Inhibitor, Clade A, Member 11

Entrez Gene IDs

256394 (Human); 380780 (Mouse); 362774 (Rat)

Alternate Names

Antiproteinase-Like 2; antitrypsin), member 11; member 11; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A11; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)