Human Serpin A4/Kallistatin Antibody

Catalog #: MAB1669 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1669
MAB1669-SP

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Human Serpin A4/Kallistatin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Serpin A4/Kallistatin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Serpin A1, A3, A5, C1, F2, or recombinant mouse Serpin D1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 209930

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin A4/Kallistatin
Leu22-Pro427
Accession # AAA59454

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Serpin A4/Kallistatin (Catalog # 1669-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A4/Kallistatin

Serpin A4, also called Kallistatin, Kallikrein inhibitor and protease inhibitor 4, is a member of the Serpin superfamily of the serine protease inhibitors (1). Known also as kallistatin or proteinase inhibitor 4, Serpin A4 has been shown to function as a specific inhibitor of tissue kallikrein and as an inhibitor of angiogenesis and tumor growth (2, 3).

References

Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
Chen, V.C. et al. (2000) J. Biol. Chem. 275:40371.
Miao, R.Q. et al. (2002) Blood 100:3245.

Entrez Gene IDs

5267 (Human)

Alternate Names

antitrypsin), member 4; KAL; Kallistatin; KLST; KSTKallikrein inhibitor; member 4; PI-4; PI4Peptidase inhibitor 4; protease inhibitor 4 (kallistatin); serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A4; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin)