Home / Serpin A5/Protein C Inhibitor / Human Serpin A5/Protein C Inhibitor Antibody

Human Serpin A5/Protein C Inhibitor Antibody

Catalog #: MAB1266
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1266
MAB1266-SP
Detection of Serpin A5 in HepG2 Human Cell Line by Flow Cytometry.
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Serpin A5/Protein C Inhibitor Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin A5/Protein C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Serpin A1, A3, F2, C1, or recombinant mouse Serpin A5 is observed.
Source
Monoclonal Mouse IgG2A Clone # 210326
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin A5/Protein C
His20-Pro406
Accession # P05154
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Serpin A5/Protein C Inhibitor (Catalog # 1266-PI)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Serpin A5/
Protein C Inhibitor (Catalog # 1266-PI), see our available Western blot detection antibodies
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Serpin A5 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Serpin A5 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serpin A5 Monoclonal Antibody (Catalog # MAB1266, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A5/Protein C Inhibitor

Serpin A5 is the a member of the Serpin superfamily and inhibits a variety of serine proteases such as protein C, plasminogen activators, thrombin, factor Xa, several kallekreins and acrosin (1). Serpin A5 is synthesized in the liver and secreted in plasma. It is found in numerous steroid-responsive organs and has been detected in saliva, cerebral spinal fluid, amniotic fluid, tears and semen. Because of its protease targets and regulated expression patterns, Serpin A5 has been proposed to play a role in processes such as blood coagulation, fertilization and carcinogenesis (2, 3). Similar to Serpins C1 and D1, its thrombin inhibitory activity is enhanced by heparin.

References
  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Palmier, D. et al. (2002) J. Biol. Chem. 277:40950.
  3. Geiger, M. et al. (1996) Immunopharmacology 32:53.
Entrez Gene IDs
5104 (Human); 268591 (Mouse)
Alternate Names
Acrosomal serine protease inhibitor; antitrypsin), member 5; member 5; PAI-3; PAI3PLANH3; PCI; PCIplasminogen activator inhibitor III; Plasminogen activator inhibitor 3; plasminogen activator inhibitor-3; PROCIplasma serine protease inhibitor; Protein C inhibitor; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A5; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); SERPNA5

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Citations for Human Serpin A5/Protein C Inhibitor Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Transcriptomic analysis to identify genes associated with selective hippocampal vulnerability in Alzheimer's disease
    Authors: AM Crist, KM Hinkle, X Wang, CM Moloney, BJ Matchett, SA Labuzan, I Frankenhau, NO Azu, AM Liesinger, ER Lesser, DJ Serie, ZS Quicksall, TA Patel, TP Carnwath, M DeTure, X Tang, RC Petersen, R Duara, NR Graff-Radf, M Allen, MM Carrasquil, H Li, OA Ross, N Ertekin-Ta, DW Dickson, YW Asmann, RE Carter, ME Murray
    Nature Communications, 2021-04-19;12(1):2311.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Co-IP, IHC, Western Blot
  2. Interaction of protein C inhibitor with the type II transmembrane serine protease enteropeptidase.
    Authors: Prohaska TA, Wahlmuller FC, Furtmuller M, Geiger M
    PLoS ONE, 2012-06-19;7(6):e39262.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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