Human Serpin B8/Proteinase Inhibitor 8 Antibody

Catalog #: AF4158 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4158
AF4158-SP

All Serpin B8/Proteinase Inhibitor 8 Antibodies

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Human Serpin B8/Proteinase Inhibitor 8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Serpin B8/Proteinase Inhibitor 8 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse Serpin B8 is observed and less than 2% cross-reactivity with recombinant human (rh) Serpin B6, rhSerpin B9, and rhSerpin A5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin B8/Proteinase Inhibitor 8
Asp2-Pro374
Accession # P50452

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Serpin B8/Proteinase Inhibitor 8

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Serpin B8/Proteinase Inhibitor 8, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin B8/Proteinase Inhibitor 8

Serpin B8 (serine proteinase inhibitor‑clade B #8; also PI8) is a 42‑45 kDa, cytoplasmic and secreted member of the ovalbumin (clade B)‑subfamily, Serpin superfamily of protease inhibitors. It is produced by multiple cell types and shows inhibitory activity towards furin and other proteinases such as chymotrypsin. Human Serpin B8 is 374 amino acids (aa) in length. Although it has no signal sequence, it is released by platelets. The active inhibitor site lies between Arg336 and Arg342. One potential isoform shows a 2 aa substitution for the C‑terminal 134 amino acids. Full length human Serpin B8 shares 78% and 83% aa identity with mouse and canine Serpin B8, respectively.

Entrez Gene IDs

5271 (Human); 20725 (Mouse); 288937 (Rat)

Alternate Names

CAP2; CAP-2; CAP2clade B (ovalbumin), member 8; Cytoplasmic antiproteinase 2; NK10; Peptidase inhibitor 8; PI8; PI-8; protease inhibitor 8 (ovalbumin type); Serpin B8; serpin peptidase inhibitor, clade B (ovalbumin), member 8