Human Serpin D1/Heparin Cofactor II Biotinylated Antibody

Catalog #: BAF3198 Datasheet / COA / SDS

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BAF3198

All Serpin D1/Heparin Cofactor II Antibodies

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Human Serpin D1/Heparin Cofactor II Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Serpin D1/Heparin Cofactor II in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse Serpin D1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin D1/Heparin Cofactor II
Gly20-Arg498
Accession # P05546

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Serpin D1/Heparin Cofactor II (Catalog # 3198-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin D1/Heparin Cofactor II

Serpin D1, also known as Heparin Cofactor II, is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3). The full-length human cDNA was expressed and the purified protein corresponded to the secreted form with anti-thrombin activity.

Entrez Gene IDs

3053 (Human); 15160 (Mouse)

Alternate Names

HC2; HC2LS2; HCF2; HCF2clade D (heparin cofactor), member 1; HC-II; Heparin Cofactor II; HLS2HCII; leuserpin 2; LS2; Protease inhibitor leuserpin-2; Serpin D1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1