Home / Serpin F1/PEDF / Human Serpin F1/PEDF Antibody

Human Serpin F1/PEDF Antibody

Catalog #: MAB1177
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1177-SP
MAB1177-500
MAB1177-100
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Serpin F1/PEDF Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin F1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Serpin F1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 187003
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Serpin F1, Mouse myeloma cell line NS0-derived recombinant human Serpin F1, and S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin F1
Asp44-Pro418
Accession # P36955
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Serpin F1/PEDF
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human skin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin F1/PEDF

Serpin F1, also known as pigment epithelium derived factor (PEDF), is a secreted glycoprotein that shows sequence and structural similarity with the serpin superfamily proteins, but lacks serine protease inhibitory activity. Serpin F1 has been shown to have cell-type specific functions including inhibition of retinal angiogenesis and neurotrophic activity in the retina and central nervous system.

Long Name
Serpine F1/Pigment Epithelium-derived Factor
Entrez Gene IDs
5176 (Human); 20317 (Mouse); 287526 (Rat)
Alternate Names
Cell proliferation-inducing gene 35 protein; EPC-1; EPC-1PIG35; PEDF; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1

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Citations for Human Serpin F1/PEDF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Attenuation of choroidal neovascularization by histone deacetylase inhibitor.
    Authors: Chan N, He S, Spee C, Ishikawa K, Hinton D
    PLoS ONE, 2015-03-25;10(3):e0120587.
  2. Systemically administered liposome-encapsulated Ad-PEDF potentiates the anti-cancer effects in mouse lung metastasis melanoma.
    Authors: Shi, Hua-shan, Yang, Li-ping, Wei, Wei, Su, Xiao-qin, Li, Xiao-pen, Li, Meng, Luo, Shun-tao, Zhang, Hai-long, Lu, Lian, Mao, Yong-qiu, Kan, Bing, Yang, Li
    J Transl Med, 2013-04-03;11(0):86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  3. Inhibition of Angiogenesis by HC·HA, a Complex of Hyaluronan and the Heavy Chain of Inter-alpha -Inhibitor, Purified from Human Amniotic Membrane
    Authors: Elizabeth Shay, Hua He, Shunsuke Sakurai, Scheffer C. G. Tseng
    Investigative Opthalmology & Visual Science

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