Human Serum Albumin Antibody

Catalog #: MAB14551 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB14551-100
MAB14551-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Serum Albumin Antibody Summary

Species Reactivity

Human

Specificity

Detects Human Serum Albumin in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 188815

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human Serum Albumin

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB14552).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Serum Albumin DuoSet ELISA Kit (Catalog # DY1455) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Albumin ELISA Standard Curve. Recombinant Human Albumin protein was serially diluted 2-fold and captured by Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB14552) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB14551) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Albumin

Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.

Entrez Gene IDs

213 (Human); 11657 (Mouse); 24186 (Rat)

Alternate Names

ALB; Albumin; cell growth inhibiting protein 42; DKFZp779N1935; growth-inhibiting protein 20; PRO0883; PRO0903; PRO1341; serum albumin