Human Serum Albumin APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Albumin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serum Albumin APC-conjugated Monoclonal Antibody (Catalog # IC1455A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of Albumin in THP-1 Human Cell Line by Flow Cytometry THP-1 human monocytic leukemia cell line (negative control) was stained with Mouse Anti-Human Serum Albumin APC-conjugated Monoclonal Antibody (Catalog # IC1455A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Albumin
Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.
Product Datasheets
Citations for Human Serum Albumin APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Spectroscopic label-free microscopy of changes in live cell chromatin and biochemical composition in transplantable organoids
Authors: G Pettinato, MF Coughlan, X Zhang, L Chen, U Khan, M Glyavina, CJ Sheil, PK Upputuri, YN Zakharov, E Vitkin, AB D'Assoro, RA Fisher, I Itzkan, L Zhang, L Qiu, LT Perelman
Science Advances, 2021-08-18;7(34):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells
Authors: G Pettinato, S Lehoux, R Ramanathan, MM Salem, LX He, O Muse, R Flaumenhaf, MT Thompson, EA Rouse, RD Cummings, X Wen, RA Fisher
Sci Rep, 2019-06-20;9(1):8920.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Recapitulation of methotrexate hepatotoxicity with induced pluripotent stem cell-derived hepatocytes from patients with rheumatoid arthritis
Authors: J Kim, Y Kim, J Choi, H Jung, K Lee, J Kang, N Park, YA Rim, Y Nam, JH Ju
Stem Cell Res Ther, 2018-12-29;9(1):357.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Scalable Differentiation of Human iPSCs in a Multicellular Spheroid-based 3D Culture into Hepatocyte-like Cells through Direct Wnt/?-catenin Pathway Inhibition
Sci Rep, 2016-09-12;6(0):32888.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
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What is the light chain of Human Serum Albumin Antibody, Catalog #s MAB1455 and IC1455, Clone #188835?
Catalog #s MAB1455 and IC1455 have a kappa light chain.
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