Human sFRP-1 Biotinylated Antibody Summary
Ser32-Lys314
Accession # AAB70793
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: sFRP-1
Secreted Frizzled Related Proteins (sFRPs) are a family of secreted, soluble vertebrate glycoproteins which contain homology to the Wnt-binding domain of the Frizzled (Fz) family of transmembrane receptors. sFRPs are approximately 30 - 35 kDa in size and are comprised of 3 domains: a signal sequence; an N-terminal Fz cysteine-rich domain (CRD) with 10 conserved cysteines; and a C-terminal heparin-binding region with weak homology to Netrin. The Fz CRD mediates Wnt-binding and is present in all Fz and sFRP family members (1).
sFRP-1, also known as secreted apoptosis-related protein 2 (SARP-2), FRP and FrzA, is expressed in the embryonic kidney, eye, brain, teeth, salivary gland and small intestine, most often at sites of epithelial-mesenchyme interaction (5). Expression in the adult animal is strong in the eye, kidney, and heart and also prevalent in the brain and lung (2, 5). sFRP-1 was first characterized as a protein that enhances the sensitivity of cells to apoptotic stimuli (3) and as an antagonist of Wnt signaling in Xenopus embryos (4). It is also characterized as a tumor suppressor in breast (6) and cervical carcinomas (7). In contrast, sFRP-1 is expressed by the majority of malignant gliomas (8) and contributes to the development of uterine leiomyomas (9), suggesting that the role of sFRP-1 is dependent on cell context. sFRP-1 has diverse activities, from inducing angiogenesis (10) in a variety of in vivo models to helping regulate Wnt-4 signaling (with sFRP-2) in renal organogenesis (11). Mouse and human sFRP-1 proteins share 94% amino acid identity (1).
- Jones, S. et al. (2002) Bioessays 24:811.
- Rattner, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:2859.
- Melkonyan, H. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13636.
- Finch, P. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6770.
- Leimeister, C. et al. (1998) Mech. Dev. 75:29.
- Ugolini, F. et al. (2001) Oncogene 20:5810.
- Ko, J. et al. (2002) Exp. Cell. Res. 280:280.
- Roth, W. et al. (2000) Oncogene 19:4210.
- Fukuhara, K, et al. (2002) J. Clin. Endocr. Metab. 87:1729.
- Dufourcq, P. et al. (2002) Circulation 106: 3097.
- Yoshino, K. et al. (2001) Mech. Dev. 102:45.
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