Human SH2D1A Antibody

Catalog #: AF7440 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7440
AF7440-SP

Detection of Human SH2D1A by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SH2D1A Antibody Summary

Species Reactivity

Human

Specificity

Detects human SH2D1A in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human SH2D1A
Met1-Pro128
Accession # O60880

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SH2D1A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SH2D1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7440) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SH2D1A at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SH2D1A

SH2D1A (Src-Homology 2 Domain-Containing protein A1; also SAP and DSHP) is a 15-19 kDa protein that represents the product of the XLP (X-linked Lymphoproliferative Disease) gene. It is a cytoplasmic protein that is expressed in T cells, NK cells, and memory B cells. SH2D1A interacts with the SH2 binding domains of at least six SLAM family cell membrane proteins, including Ly9, CD84, 2B4/CD244, NTBA, CS1 and SLAM/CD150. This has the effect of blocking an SHP2 interaction with the above phosphorylated receptors and abrogating downstream signaling. Notably, SH2D1A also recruits and activates the Src kinase Fyn when bound to SLAM family molecules, and this action presumably impacts additional downstream signaling. Human SH2D1A is 128 amino acids (aa) in length. Although it contains only one SH2 domain (aa 6-104), this domain can be utilized simultaneously by SLAM and Fyn, creating a trimeric complex. There are multiple splice variants for SH2D1A. One shows a three aa deletion of aa 114-116, a second possesses a nine aa substitution for aa 68-128, a third contains a 16 aa substitution for aa 47-128, a fourth possesses a 12 aa substitution for aa 47-128, while a fifth contains a 37 aa substitution for aa 40-128. Full length human SH2D1A shares 88% aa identity with mouse SH2D1A.

Entrez Gene IDs

4068 (Human); 20400 (Mouse); 501502 (Rat)

Alternate Names

DSHP; DSHPLYP; Duncan disease SH2-protein; EBVS; FLJ92177; IMD5; LYP; MTCP1; SAP/SH2D1A; SAPlymphoproliferative syndrome; SH2 domain containing 1A; SH2 domain-containing protein 1A; SH2D1A; signaling lymphocyte activation molecule-associated protein; Signaling lymphocytic activation molecule-associated protein; SLAM associated protein/SH2 domain protein 1A; SLAM-associated protein; T cell signal transduction molecule SAP; T-cell signal transduction molecule SAP; XLP; XLPD; XLPDFLJ18687; XLPSH2 domain protein 1A