What is the normal appearance of Assay Diluent RD1-75?

Name: Human SHBG Quantikine ELISA Kit
Brand: R&D Systems
SKU: DSHBG0B
Rating: 5 (2 reviews)

Assay Diluent RD1-75 contains high concentrations of additives and typically has a very cloudy, brown appearance. It is recommended to mix this diluent well before and during use.

Human SHBG ELISA Kit - Quantikine

Catalog #: DSHBG0B
9 Citations 2 Reviews Datasheet / COA / SDS

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DSHBG0B

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Product Details

Citations (9)

FAQs

Reviews (2)

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human SHBG Quantikine ELISA Kit Summary

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.012 nmol/L

Assay Range

0.1 - 4 nmol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human SHBG.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine^® Human SHBG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SHBG in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human SHBG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SHBG showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human SHBG.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using one lot of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (nmol/L)	0.565	1.36	2.72	0.562	1.35	2.71
Standard Deviation	0.032	0.04	0.097	0.042	0.079	0.131
CV%	5.7	3	3.6	7.6	5.8	4.8

Recovery

The recovery of human SHBG spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human SHBG were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human SHBG Standard Curve

N/A

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Human SHBG ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SHBG

SHBG (Sex Hormone-binding Globulin), also known as ABP (Androgen-binding Protein), is a variably glycosylated, secreted, non-disulfide-linked homodimer. Male and female sex hormones bind SHBG with equal affinity. This binding protein can be found in the circulation where it regulates hormone access to target tissues. Human SHBG shares approximately 67% amino acid sequence identity with mouse and rat SHBG.

Long Name:

Sex Hormone-binding Globulin

Entrez Gene IDs:

6462 (Human)

Alternate Names:

ABP; ABPTestosterone-estrogen-binding globulin; androgen binding protein; MGC126834; MGC138391; SBP; sex hormone-binding globulin; Sex steroid-binding protein; SHBG; TEBG; Testis-specific androgen-binding protein; testosterone-binding beta-globulin; Testosterone-estradiol-binding globulin

Related Research Areas

Steroid Hormones and Bioactive Lipids

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human SHBG Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Shorter androgen receptor polyQ alleles protect against life-threatening COVID-19 disease in European males
Authors: M Baldassarr, N Picchiotti, F Fava, C Fallerini, E Benetti, S Daga, F Valentino, G Doddato, S Furini, A Giliberti, R Tita, S Amitrano, M Bruttini, S Croci, I Meloni, AM Pinto, N Iuso, C Gabbi, F Sciarra, MA Venneri, M Gori, M Sanarico, FP Crawley, U Pagotto, F Fanelli, M Mezzullo, E Dominguez-, L Planas-Ser, A Schlüter, R Colobran, P Soler-Pala, P Lapunzina, J Tenorio, A Pujol, MG Castagna, M Marcelli, AM Isidori, A Renieri, E Frullanti, F Mari, GEN-COVID Multicenter Stu Spanish Co
EBioMedicine, 2021-02-26;65(0):103246.
Species: Human
Sample Types: Plasma
Maternal metabolic profiling to assess fetal gestational age and predict preterm delivery: a two-centre retrospective cohort study in the US
Authors: KG Sylvester, S Hao, J You, L Zheng, L Tian, X Yao, L Mo, S Ladella, RJ Wong, GM Shaw, DK Stevenson, HJ Cohen, JC Whitin, DB McElhinney, XB Ling
BMJ Open, 2020-12-02;10(12):e040647.
Species: Human
Sample Types: Serum
Pregnancy outcome in women with polycystic ovary syndrome in relation to second-trimester testosterone levels
Authors: R Valdimarsd, AK Wikström, TK Kallak, E Elenis, O Axelsson, H Preissl, SJKA Ubhayaseke, J Bergquist, IS Poromaa
Reprod Biomed Online, 2020-09-26;0(0):.
Species: Human
Sample Types: Plasma
Hormonal determinants of mammographic density and density change
Authors: M Gabrielson, S Azam, E Hardell, M Holm, KA Ubhayaseke, M Eriksson, M Bäcklund, J Bergquist, K Czene, P Hall
Breast Cancer Res., 2020-08-26;22(1):95.
Species: Human
Sample Types: Plasma
Assessing C reactive protein/albumin ratio as a new biomarker for polycystic ovary syndrome: a case-control study of women from Bahraini medical clinics
Authors: S Kalyan, A Goshtesabi, S Sarray, A Joannou, WY Almawi
BMJ Open, 2018-10-27;8(10):e021860.
Species: Human
Sample Types: Serum
Sex hormone-binding globulin (SHBG) is a potential early diagnostic biomarker for gastric cancer
Authors: CW Cheng, CC Chang, YN Patria, RT Chang, YR Liu, FA Li, HM Shih, CY Lin
Cancer Med, 2017-11-17;0(0):.
Species: Human
Sample Types: Plasma
Analysis of VEGFA Variants and Changes in VEGF Levels Underscores the Contribution of VEGF to Polycystic Ovary Syndrome
PLoS ONE, 2016-11-15;11(11):e0165636.
Species: Human
Sample Types: Serum
Alterations of bone density, microstructure, and strength of the distal radius in male patients with rheumatoid arthritis: a case-control study with HR-pQCT.
Authors: Zhu T, Griffith J, Qin L, Hung V, Fong T, Au S, Li M, Lam Y, Wong C, Kwok A, Leung P, Li E, Tam L
J Bone Miner Res, 2014-09-01;29(9):2118-29.
Species: Human
Sample Types: Serum
A randomized phase II presurgical trial of transdermal 4-hydroxytamoxifen gel versus oral tamoxifen in women with ductal carcinoma in situ of the breast.
Authors: Lee O, Page K, Ivancic D, Helenowski I, Parini V, Sullivan M, Margenthaler J, Chatterton R, Jovanovic B, Dunn B, Heckman-Stoddard B, Foster K, Muzzio M, Shklovskaya J, Skripkauskas S, Kulesza P, Green D, Hansen N, Bethke K, Jeruss J, Bergan R, Khan S
Clin Cancer Res, 2014-07-15;20(14):3672-82.
Species: Human
Sample Types: Plasma

FAQs

What is the normal appearance of Assay Diluent RD1-75?
- Assay Diluent RD1-75 contains high concentrations of additives and typically has a very cloudy, brown appearance. It is recommended to mix this diluent well before and during use.

View all ELISA FAQs