Human SHP-1 Antibody Summary
Ala205-Lys595
Accession # P29350
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![SHP-1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). SHP-1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).](https://resources.rndsystems.com/images/datasheets/antibody/SHP1_MAB1878_Immunocytochemistry__Immunofluorescence_19404.jpg)
SHP‑1 in HeLa Human Cell Line. SHP-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rat Anti-Human SHP-1 Monoclonal Antibody (Catalog # MAB1878) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SHP-1
Src-Homology 2 domain Phosphatase-1 (SHP-1), also known as Protein Tyrosine Phosphatase 1C (PTP1C), PTPN6, and Hematopoetic Cell Phosphatase (HCPH), is an enzyme that selectively dephosphorylates tyrosine residues in proteins. Spontaneous point mutations in the SHP-1 gene in mice produce the “motheaten” and "motheaten viable” phenotypes that are severely autoimmune and immunodeficient (1). The enzyme is highly expressed in leukocyte cell types (2). SHP-1 has a regulatory region containing two Src homology 2 (SH2) domains that are critical for its binding to ITIM domains in inhibitory immunoreceptors (3). Deletion of the SH2 domains, as in this product, causes a marked increase in phosphatase activity (4). SHP-1 will dephosphorylate a wide variety of proteins, including the EGF receptor (5). A phosphopeptide containing the EGFR (Y992) sequence (R&D Systems, Catalog # ES006) can be used to measure the activity of SHP-1 by detecting the release of phosphate (R&D Systems, Catalog # DY996).
- Tsui, H.W. et al. (1993) Nature Genet. 4:124.
- Matthews, R.J. et al. (1992) Mol. Cell. Biol. 12:2396.
- Burshtyn, D.N. et al. (1997) J. Biol. Chem. 272:13066.
- Pei, D. et al. (1994) Biochemistry 33:15483.
- Tomic, S. et al. (1995) J. Biol. Chem. 270:21277.
Product Datasheets
Citations for Human SHP-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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The oncoprotein gankyrin promotes the development of colitis-associated cancer through activation of STAT3
Authors: T Sakurai, H Higashitsu, H Kashida, T Watanabe, Y Komeda, T Nagai, S Hagiwara, M Kitano, N Nishida, T Abe, H Kiyonari, K Itoh, J Fujita, M Kudo
Oncotarget, 2017-04-11;8(15):24762-24776.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue.
Authors: Deschaseaux F, Gaillard J, Langonne A, Chauveau C, Naji A, Bouacida A, Rosset P, Heymann D, De Pinieux G, Rouas-Freiss N, Sensebe L
FASEB J, 2013-04-16;27(8):2977-87.
Species: Human
Sample Types: Whole Cells
Applications: ChIP
FAQs
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Reconstitution Buffers
Secondary Antibodies
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