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Human SIGIRR Antibody

Catalog #: AF990
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF990
AF990-SP
Product Details
Citations (4)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SIGIRR Antibody Summary

Species Reactivity
Human
Specificity
Detects human SIGIRR in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) IL‑1 R9, rhIL-1 R1, rhIL-1 R2, rhIL-1 R3, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, and rhIL-18 R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SIGIRR
Met1-His118
Accession # Q6IA17
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human SIGIRR Fc Chimera (Catalog # 990-SG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIGIRR

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL1RAPL1), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Five of the IL-1 R family members (1, 2, 4, 5, and 6) are clustered and localized to chromosome 2. SIGIRR (single Ig domain containing IL-1 receptor-related molecule) is a subtype of the IL-1 R family that differs from the other nine members by having only one Ig domain in its extracellular region. The sequence of human SIGIRR predicts a 410 amino acid (aa) residue transmembrane glycoprotein that lacks signal peptide and contains a 118 aa single Ig extracellular domain, a transmembrane region and a 268 aa cytoplasmic tail with a TIR domain. The cytoplasmic tail of SIGIRR contains a C-terminal extention beyond the TIR domain which is also found in IL1RAPL1, IL-1 R9, and Toll-like receptor family members but absent in other IL-1 receptor family members. SIGIRR is widely expressed and is present in all cells and tissues examined. Mouse and human SIGIRR share 82% amino acid sequence identity. The ligand and signaling mechanism for SIGIRR has not been identified.

References
  1. Thomassen, E. et al. (1999) Cytokine 11:389.
Long Name
Single lg IL-1 Related Protein
Entrez Gene IDs
59307 (Human); 24058 (Mouse)
Alternate Names
IL-1R8; SIGIRR; single Ig IL-1-related receptor; Single Ig IL-1R-related molecule; single immunoglobulin and toll-interleukin 1 receptor (TIR) domain; single immunoglobulin domain IL1R1 related; Single immunoglobulin domain-containing IL1R-related protein; TIR8; TIR8MGC110992; Toll/interleukin-1 receptor 8

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Citations for Human SIGIRR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. A Splice Switch in SIGIRR Causes a Defect of IL-37-Dependent Anti-Inflammatory Activity in Cystic Fibrosis Airway Epithelial Cells
    Authors: K Ueno-Shuto, S Kamei, M Hayashi, A Fukuyama, Y Uchida, N Tokutomi, MA Suico, H Kai, T Shuto
    International Journal of Molecular Sciences, 2022-07-13;23(14):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  2. Staphylococcus aureus intra-mammary infection affects the expression pattern of IL-R8 in goat
    Authors: J Filipe, V Bronzo, G Curone, B Castiglion, D Vigo, B Smith, V Herrera, P Roccabianc, P Moroni, F Riva
    Comp. Immunol. Microbiol. Infect. Dis., 2019-07-30;66(0):101339.
    Species: Goat
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Lipopolysaccharide decreases single immunoglobulin interleukin-1 receptor-related molecule (SIGIRR) expression by suppressing specificity protein 1 (Sp1) via the Toll-like receptor 4 (TLR4)-p38 pathway in monocytes and neutrophils.
    Authors: Ueno-Shuto K, Kato K, Tasaki Y, Sato M, Sato K, Uchida Y, Sakai H, Ono T, Suico M, Mitsutake K, Tokutomi N, Kai H, Shuto T
    J Biol Chem, 2014-05-12;289(26):18097-109.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. The single IgG IL-1-related receptor controls TLR responses in differentiated human intestinal epithelial cells.
    Authors: Khan MA, Steiner TS, Sham HP, Bergstrom KS, Huang JT, Assi K, Salh B, Tai IT, Li X, Vallance BA
    J. Immunol., 2010-02-03;184(5):2305-13.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr

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