Human Sirtuin 2/SIRT2 Biotinylated Antibody

Catalog #: BAF4358
2 Citations Datasheet / COA / SDS

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BAF4358

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Sirtuin 2/SIRT2 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Sirtuin 2/SIRT2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human SIRT1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Sirtuin 2/SIRT2
Ala2-Gln389
Accession # Q8IXJ6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sirtuin 2/SIRT2

Sirtuin 2, encoded by the SIRT2 gene, is also known as SIR2 (silent information regulator 2)-like protein 2. It is a nicotinamide adenine dinucleotide (NAD)-dependent histone/protein deacetylase (1, 2). The SIR2 family of enzymes is classified as class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging (3, 4). Unlike class I and class II HDACs, the enzymatic activity of class III HDACs is NAD dependent and insensitive to HDAC inhibitor trichostatin A (5).

Long Name

Silent Mating Type Information Regulation 2 Homolog

Entrez Gene IDs

22933 (Human); 64383 (Mouse); 361532 (Rat)

Alternate Names

EC 3.5.1; EC 3.5.1.-; FLJ35621; FLJ37491; S.cerevisiae, homolog) 2; SIR2L2; SIRT2; Sirtuin 2; sirtuin type 2

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human Sirtuin 2/SIRT2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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