Human SLC7A5/LAT1 Antibody

Catalog #: MAB10390 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10390-100
MAB10390-SP

Detection of SLC7A5/LAT1 in Raji Human Cell Line by Flow Cytometry.

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Human SLC7A5/LAT1 Antibody Summary

Species Reactivity

Human

Specificity

Detects Human SLC7A5/LAT1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # BU53

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Plasma cell line 8226
Accession # Q01650

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of SLC7A5/LAT1 in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt lymphoma cell line was stained with Mouse Anti-Human SLC7A5/LAT1 Monoclonal Antibody (Catalog # MAB10390, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLC7A5/LAT1

Human Large neutral amino acids transporter small subunit 1, also known as SLC7A5, CD98 Light Chain (CD98LC), 4F2 light chain and LAT1 belongs to the APC (Amino acid-Polyamine-organo Cation) superfamily and forms a heterodimer with SLC3A2 to function as sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, L-DOPA, leucine, histidine, methionine and tryptophan.

Long Name

Solute Carrier Family 7 Member 5

Entrez Gene IDs

8140 (Human); 20539 (Mouse); 50719 (Rat)

Alternate Names

4F2 LC; 4F2LC; CD98; CD98lc; D16S469EL-type amino acid transporter 1; E16; Integral membrane protein E16; large neutral amino acids transporter 1; large neutral amino acids transporter small subunit 1; LAT1; LAT1hLAT1; MPE16; MPE16CD98 light chain; SLC7A5; sodium-independent neutral amino acid transporter LAT1,4F2LC; solute carrier family 7 (cationic amino acid transporter, y+ system), member 5,4F2 light chain; Solute carrier family 7 member 5; TA1; y+ system cationic amino acid transporter