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Human SLPI Antibody

Catalog #: MAB1274
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1274
MAB1274-SP
Product Details
Citations (4)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SLPI Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human SLPI in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant mouse SLPI is observed.
Source
Monoclonal Mouse IgG1 Clone # 20409
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SLPI
Accession # P03973
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human SLPI (Catalog # 1274-PI) under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLPI

SLPI is a member of the Serpin family and is primarily expressed in secretory glandular epithelial cells. It is found in various secretory fluids and functions as a potent inhibitor of many proteolytic enzymes. SLPI can modulate cell proliferation and has been shown to have anti-inflammatory, anti-viral, and anti-bacterial activities (1, 2).

References
  1. Zhang, D. et al. (2002) J. Biol. Chem. 277:29999.
  2. Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.
Long Name
Secretory Leukocyte Protease Inhibitor
Entrez Gene IDs
6590 (Human); 20568 (Mouse)
Alternate Names
ALK1; ALPProtease inhibitor WAP4; antileukoproteinase; BLPISeminal proteinase inhibitor; HUSI; HUSI-I; Mucus proteinase inhibitor; secretory leukocyte peptidase inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase); Secretory leukocyte protease inhibitor; SLPI; WAP four-disulfide core domain protein 4; WAP4HUSI-1; WFDC4MPI

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Citations for Human SLPI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Endogenous secretory leukocyte protease inhibitor (SLPI) inhibits microbial-induced monocyte activation
    Authors: S Nugteren, Y Simons-Oos, CL Menckeberg, DH Hulleman-v, DJ Lindenberg, JN Samsom
    European Journal of Immunology, 2022-12-22;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  2. Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach.
    Authors: Gronborg M, Kristiansen TZ, Iwahori A, Chang R, Reddy R, Sato N, Molina H, Jensen ON, Hruban RH, Goggins MG, Maitra A, Pandey A
    Mol. Cell Proteomics, 2005-10-08;5(1):157-71.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions
    Authors: Austin W. Boesch, Yifeng Zhao, Alison S. Landman, Marta Rodriguez Garcia, John V. Fahey, Charles R. Wira et al.
    Journal of Immunological Methods
  4. An efficient power harvesting mobile phone-based electrochemical biosensor for point-of-care health monitoring
    Authors: Alexander C. Sun, Chengyang Yao, Venkatesh Venkatesh, Drew A. Hall
    Sensors and Actuators B: Chemical

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