Human Smoothelin B Antibody Summary
Met1-Leu132
Accession # P53814
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Smoothelin B in Human Colon. Smoothelin B was detected in formalin fixed paraffin-embedded sections of human colon using Mouse Anti-Human Smoothelin B Monoclonal Antibody (Catalog # MAB8278) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells in vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Smoothelin in HASMC Human Cells by Flow Cytometry. HASMC human aortic smooth muscle cells was stained with Mouse Anti-Human Smoothelin B Monoclonal Antibody (Catalog # MAB8278, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Smoothelin
Smoothelin (SMTN) is a cytoskeletal protein associated with stress fibers. It is 917 amino acids (aa) in length and shares 77% and 82% aa identity with mouse and rat SMTN. SMTN has two major isoforms, A and B with reported molecular weights of approximately 59 and 110 kDa, respectively. Isoform A is associated with visceral smooth muscle cells while isoform B is specific to vascular smooth muscle. Loss of SMTN has been associated with defects in intestinal smooth muscle cell contraction as well as increased arterial pressure and cardiac hypertrophy.
Product Datasheets
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A wide titration of this Ab was tested on human aortic SMC (Lonza) using an Intercellular Staining Kit (Life Sciences). This Ab was tested negative even though the SMC were stained highly positive for CNN1 (Sigma). Although this Ab did not work, R&D customer service was very helpful and sent a replacement Ab.
