Human Snail Alexa Fluor® 488-conjugated Antibody

Catalog #: IC3639G Datasheet / COA / SDS

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IC3639G-025

Detection of Snail in A549 Human Cell Line by Flow Cytometry.

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Human Snail Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Snail in direct ELISAs.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Snail
Pro2-Arg264
Accession # O95863

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

5 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Snail antibody in A549 Human Cell Line antibody by Flow Cytometry.

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Detection of Snail in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Goat Anti-Human Snail Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC3639G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Snail

Snail is predicted 29 kDa nuclear zinc finger transcriptional repressor that contains an N-terminal basic SNAG domain followed by three classical and one atypical zinc finger domains. During development, Snail is required for the establishment of left-right axis asymmetry. It also regulates the transcription of E-cadherin and other genes involved in epithelial-mesenchymal transitions during cancer progression. Human Snail shares 88% amino acid sequence identity with mouse and rat Snail.

Entrez Gene IDs

6615 (Human); 20613 (Mouse); 116490 (Rat)

Alternate Names

dJ710H13.1; Protein sna; Protein snail homolog 1; SLUGH2; SNA; SNAH; SNAI1; snail 1 homolog; snail 1 zinc finger protein; snail family zinc finger 1; snail homolog 1; Snail; SNAIL1; zinc finger protein SNAI1