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Human Somatostatin R2/SSTR2 Antibody

Catalog #: MAB4224
14 Citations Datasheet / COA / SDS
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MAB4224
MAB4224-SP

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Somatostatin R2/SSTR2 in MDA-MB-231 Human Cell Line.
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Citations (14)
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Human Somatostatin R2/SSTR2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Somatostatin R2/SSTR2. Stains SSTR2 transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 402038
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with Somatostatin R2/SSTR2
Met1-Ile369
Accession # P30874
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Somatostatin R2/SSTR2 antibody in MDA-MB-231 Human Cell Line by Immunocytochemistry (ICC). View Larger

Somatostatin R2/SSTR2 in MDA-MB-231 Human Cell Line. Somatostatin R2/SSTR2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using 10 µg/mL Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (brown; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of Somatostatin R2/SSTR2 antibody in MDA‑MB‑231 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Somatostatin R2/SSTR2 in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Immunohistochemistry Somatostatin R2/SSTR2 antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

Somatostatin R2/SSTR2 in Human Brain. Somatostatin R2/ SSTR2 was detected in immersion fixed paraffin-embedded sections of human brain (cingulate cortex) using 3 µg/mL Human Somatostatin R2/SSTR2 Monoclonal Antibody (Catalog # MAB4224) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Somatostatin R2/SSTR2

Somatostatin Receptor 2 (SSTR2) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Human SSTR2 shares 84% aa identity with mouse SSTR2 within the extracellular domains. Isoform B (357 aa) has an alternate C-terminal cytoplasmic region that is 12 aa shorter than that of isoform A (369 aa, reported as 93 kDa). Both are expressed in brain, stomach, intestinal epithelia, pancreatic islets and kidney tubules. Isoform B is also expressed in parotid, thyroid and bronchial glands.

Long Name
Somatostatin Receptor Type 2
Entrez Gene IDs
6752 (Human); 20606 (Mouse); 54305 (Rat)
Alternate Names
Smstr2; Somatostatin R2; somatostatin receptor 2; somatostatin receptor type 2; SomatostatinR2; SRIF-1; SS2R; SS-2-R; SS2-R; SST2; SSTR2

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Citations for Human Somatostatin R2/SSTR2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Pulmonary Carcinoid Surface Receptor Modulation Using Histone Deacetylase Inhibitors
    Authors: Guenter RE, Aweda T, Carmona Matos DM et al.
    Cancers (Basel)
    Species: Human
    Sample Types:
    Applications: Flow
  2. The Wnt pathway protein Dvl1 targets Somatostatin receptor 2 for lysosome-dependent degradation
    Authors: HS Carr, Y Zuo, JA Frost
    The Journal of Biological Chemistry, 2023-03-23;0(0):104645.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Western Blot
  3. Combination of [177Lu]Lu-DOTA-TATE Targeted Radionuclide Therapy and Photothermal Therapy as a Promising Approach for Cancer Treatment: In Vivo Studies in a Human Xenograft Mouse Model
    Authors: M Simón, JT Jørgensen, HA Khare, C Christense, CH Nielsen, A Kjaer
    Pharmaceutics, 2022-06-16;14(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Development of anti-somatostatin receptors CAR T cells for treatment of neuroendocrine tumors
    Authors: Mandriani B, Pellè E, Mannavola F et al.
    Journal for immunotherapy of cancer
  5. Evaluation of Ac-Lys0(IRDye800CW)Tyr3-octreotate as a novel tracer for SSTR2-targeted molecular fluorescence guided surgery in meningioma
    Authors: Bianca M. Dijkstra, Marion de Jong, Marcus C. M. Stroet, Fritz Andreae, Sebastiaan E. Dulfer, Marieke Everts et al.
    Journal of Neuro-Oncology
  6. Primate cell fusion disentangles gene regulatory divergence in neurodevelopment
    Authors: RM Agoglia, D Sun, F Birey, SJ Yoon, Y Miura, K Sabatini, SP Pa?ca, HB Fraser
    Nature, 2021-03-17;0(0):.
    Species: Human, Primate
    Sample Types: Organoid
    Applications: ICC
  7. 177Lu-DOTA-EB-TATE, a Radiolabeled Analogue of Somatostatin Receptor Type 2, for the Imaging and Treatment of Thyroid Cancer
    Authors: Shilpa Thakur, Brianna Daley, Corina Millo, Craig Cochran, Orit Jacobson, Huiyan Lu et al.
    Clinical Cancer Research
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Overexpression of somatostatin receptor type 2 in neuroendocrine tumors for improved Ga68-DOTATATE imaging and treatment
    Authors: Rachael Guenter, Tolulope Aweda, Danilea M. Carmona Matos, Samuel Jang, Jason Whitt, Yi-Qiang Cheng et al.
    Surgery
  9. Pulmonary Carcinoid Surface Receptor Modulation Using Histone Deacetylase Inhibitors
    Authors: Rachael E. Guenter, Tolulope Aweda, Danilea M. Carmona Matos, Jason Whitt, Alexander W. Chang, Eric Y. Cheng et al.
    Cancers (Basel)
  10. Development and application of a high-content virion display human GPCR array
    Authors: Guan-Da Syu, Shih-Chin Wang, Guangzhong Ma, Shuang Liu, Donna Pearce, Atish Prakash et al.
    Nature Communications
  11. SSTR-2 as a potential tumour-specific marker for fluorescence-guided meningioma surgery
    Authors: B. M. Dijkstra, A. Motekallemi, W. F. A. den Dunnen, J. R. Jeltema, G. M. van Dam, F. A. E. Kruyt et al.
    Acta Neurochir (Wien)
  12. Targeting Somatostatin Receptors By Functionalized Mesoporous Silica Nanoparticles - Are We Striking Home?
    Authors: Valeriy M Paramonov, Diti Desai, Helene Kettiger, Veronika Mamaeva, Jessica M Rosenholm, Cecilia Sahlgren et al.
    Nanotheranostics
  13. Identification of critical residues involved in ligand binding and G protein signaling in human somatostatin receptor subtype 2.
    Authors: Parry JJ, Chen R, Andrews R
    Endocrinology, 2012-04-11;153(6):2747-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Clinical Significance of Protein Expression of Cyclooxygenase-2 and Somatostatin Receptors in Gastroenteropancreatic Neuroendocrine Tumors
    Authors: Hee Sung Kim, Hye Seung Lee, Woo Ho Kim
    Cancer Research and Treatment
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry

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