Human SOST/Sclerostin Antibody

Detection of SOST/Sclerostin by Western Blot Response of serum sclerostin and Wnt/ beta ‐catenin signaling within scWAT to 4 weeks of SIT. (a) Serum sclerostin response to SIT. (b) Representative immunoblots of sclerostin, phosphorylated and total GSK3 beta, beta ‐catenin, and total protein loading control (Ponceau S) at pre‐SIT (labeled “a”) and post‐SIT (labeled “b”) with estimated molecular weights on the right. These representative samples are the same two participants (“1” and “2”) in sequence across proteins that highlight their response from pre‐ to post‐SIT. scWAT (c) sclerostin content, (d) total and phosphorylated GSK3 beta, and (e) total beta ‐catenin response to SIT relative to pre‐SIT. Bar charts have black lines that represent individual responses to SIT with group means presented as white (pre‐SIT) and gray (post‐SIT) bars ± SD. Paired t‐tests were used to examine changes pre‐ to post‐SIT Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35312183), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SOST/Sclerostin by Western Blot Response of eWAT sclerostin content (A), serine9 phosphorylation status (B,D) both total GSK3b content (C), and total b-catenin content (E) to HFD and acute exercise, as well as representative immunoblots (F). Light grey bars represent sedentary mice and dark grey bars represent mice sampled 2 h following 2 h of acute endurance exercise and are grouped by diet; LFD = low fat diet; HFD = high fat diet. Proteins are corrected for by the house keeping protein vinculin and are all relative to LFD sedentary mice, data are presented as means ± SD and main effects are presented on graphs only if they are either close to or are significant. A two-way factorial ANOVA was used to examine main effects for diet and exercise as well their interaction. Tukey correction was used for multiple pairwise comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36685192), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SOST/Sclerostin by Western Blot Response of eWAT sclerostin content (A), serine9 phosphorylation status (B,D) both total GSK3b content (C), and total b-catenin content (E) to HFD and acute exercise, as well as representative immunoblots (F). Light grey bars represent sedentary mice and dark grey bars represent mice sampled 2 h following 2 h of acute endurance exercise and are grouped by diet; LFD = low fat diet; HFD = high fat diet. Proteins are corrected for by the house keeping protein vinculin and are all relative to LFD sedentary mice, data are presented as means ± SD and main effects are presented on graphs only if they are either close to or are significant. A two-way factorial ANOVA was used to examine main effects for diet and exercise as well their interaction. Tukey correction was used for multiple pairwise comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36685192), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SOST/Sclerostin by Western Blot Response of iWAT sclerostin content (A), serine9 phosphorylation status (B,D) both total GSK3b content (C), and total b-catenin content (E) to HFD and acute exercise, as well as representative immunoblots (F). Light grey bars represent sedentary mice and dark grey bars represent mice sampled 2 h following 2 h of acute endurance exercise and are grouped by diet; LFD = low fat diet; HFD = high fat diet. Proteins are corrected for by the house keeping protein vinculin and are all relative to LFD sedentary mice, data are presented as means ± SD and main effects are presented on graphs only if they are either close to or are significant. A two-way factorial ANOVA was used to examine main effects for diet and exercise as well their interaction. Tukey correction was used for multiple pairwise comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36685192), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of SOST/Sclerostin by Western Blot Response of iWAT sclerostin content (A), serine9 phosphorylation status (B,D) both total GSK3b content (C), and total b-catenin content (E) to HFD and acute exercise, as well as representative immunoblots (F). Light grey bars represent sedentary mice and dark grey bars represent mice sampled 2 h following 2 h of acute endurance exercise and are grouped by diet; LFD = low fat diet; HFD = high fat diet. Proteins are corrected for by the house keeping protein vinculin and are all relative to LFD sedentary mice, data are presented as means ± SD and main effects are presented on graphs only if they are either close to or are significant. A two-way factorial ANOVA was used to examine main effects for diet and exercise as well their interaction. Tukey correction was used for multiple pairwise comparisons. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36685192), licensed under a CC-BY license. Not internally tested by R&D Systems.