Human SOST/Sclerostin ELISA Kit - Quantikine

Catalog #: DSST00
12 Citations Datasheet / COA / SDS

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DSST00

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QC159: Quantikine Immunoassay Control Set797 Human SOST/Sclerostin

Human SOST/Sclerostin ELISA Standard Curve

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All SOST/Sclerostin ELISAs

Product Details

Procedure

Citations (12)

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human SOST/Sclerostin Quantikine ELISA Kit Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)

Sensitivity

3.8 pg/mL

Assay Range

31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human SOST

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human SOST immunoassay is a 4.5 hour solid-phase ELISA designed to measure SOST in human cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human SOST and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant human SOST. Results obtained using natural human SOST showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural SOST.

Recovery

The recovery of SOST spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	100	91-111
EDTA Plasma (n=4)	95	89-101
Heparin Plasma (n=4)	100	91-108
Serum (n=4)	96	86-112

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human SOST were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human SOST/Sclerostin ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SOST/Sclerostin

SOST, also known as Sclerostin, is a Cerberus/DAN family BMP antagonist and is an important regulator of bone homeostasis. Inactivating mutations in the SOST gene can cause sclerosteosis and van Buchem disease. SOST is expressed by terminally differentiated cells embedded in mineralized matrix including osteocytes, hypertrophic and prehypertrophic chondrocytes, and tooth cementocytes. Its expression is induced by BMP-2, -4, and -6 and is inhibited by parathyroid hormone (PTH). Its downregulation in osteocytes by physical loading of bone contributes to the mechanical sensor function of osteocytes and the subsequent increase in bone growth. SOST binds to BMP-2, -4, -5, -6, and -7 and inhibits the osteogenic differentiation induced by these BMPs. It inhibits canonical Wnt signaling by binding to LRP-5 and LRP-6 and inhibiting their association with Frizzled receptors. SOST reduces the proliferation of mesenchymal stem cells (MSC) and induces MSC apoptosis. Circulating levels of SOST are elevated in pathologies with bone involvement including low bone mineral density, Paget’s disease, multiple myeloma, and prostate cancer with bone metastases. It is also elevated in advanced chronic kidney disease, alcoholic liver disease, type 2 diabetes, and patients with increased abdominal and gynoid fat.

Entrez Gene IDs:

50964 (Human); 74499 (Mouse)

Alternate Names:

sclerostin; SOST; VBCHsclerosteosis

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

Prepare all reagents, standard dilutions, and samples as directed in the product insert.
Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

100 µL Assay Diluent

Add 100 µL of Assay Diluent to each well.

50 µL Standard, Control, or Sample

Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

200 µL Conjugate

Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
Aspirate and wash 4 times.

200 µL Substrate Solution

Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

50 µL Stop Solution

Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human SOST/Sclerostin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Serum granulocyte-macrophage colony-stimulating factor (GM-CSF) is increased in patients with active radiographic axial spondyloarthritis and persists despite anti-TNF treatment
Authors: C Papagoras, S Tsiami, A Chrysantho, I Mitroulis, X Baraliakos
Arthritis Research & Therapy, 2022-08-16;24(1):195.
Species: Human
Sample Types: Serum
Targeting loop3 of sclerostin preserves its cardiovascular protective action and promotes bone formation
Authors: Y Yu, L Wang, S Ni, D Li, J Liu, HY Chu, N Zhang, M Sun, N Li, Q Ren, Z Zhuo, C Zhong, D Xie, Y Li, ZK Zhang, H Zhang, M Li, Z Zhang, L Chen, X Pan, W Xia, S Zhang, A Lu, BT Zhang, G Zhang
Nature Communications, 2022-07-22;13(1):4241.
Species: Mouse, Rat
Sample Types: Serum
Baseline serum biomarkers of inflammation, bone turnover and adipokines predict spinal radiographic progression in ankylosing spondylitis patients on TNF inhibitor therapy
Authors: J Rademacher, M Siderius, L Gellert, FR Wink, M Verba, F Maas, LM Tietz, D Poddubnyy, A Spoorenber, S Arends
Seminars in arthritis and rheumatism, 2022-02-02;53(0):151974.
Species: Human
Sample Types: Serum
Assessment of Serum sRANKL, sRANKL/OPG Ratio, and Other Bone Turnover Markers with the Estimated 10-Year Risk of Major and Hip Osteoporotic Fractures in Rheumatoid Arthritis: A Cross-Sectional Study
Authors: CA Nava-Valdi, JM Ponce-Guar, AM Saldaña-Cr, EG Corona-San, M Ramirez-Vi, EE Perez-Guer, JD Murillo-Sa, B Contreras-, ML Vazquez-Vi, F Gonzalez-P, D Bonilla-La, H Jacobo-Cue, JD Centeno-Va, G Echeverria, S Cerpa-Cruz, MF Alcaraz-Lo, EG Cardona-Mu, M Salazar-Pa, L Gonzalez-L, JI Gamez-Nava
BioMed Research International, 2021-08-30;2021(0):5567666.
Species: Human
Sample Types: Serum
Comparison of insulin-like growth factor-1 and sclerostin levels between premenopausal women with and without diabetes mellitus
Authors: M Sylvawani, B Setyohadi, D Purnamasar, M Abdullah, MR Kurniawan
Journal of Taibah University Medical Sciences, 2021-06-11;16(5):719-723.
Species: Human
Sample Types: Serum
Sclerostin inhibits Wnt signaling through tandem interaction with two LRP6 ectodomains
Authors: J Kim, W Han, T Park, EJ Kim, I Bang, HS Lee, Y Jeong, K Roh, J Kim, JS Kim, C Kang, C Seok, JK Han, HJ Choi
Nat Commun, 2020-10-23;11(1):5357.
Species: Human
Sample Types: Cell Culture Supernates
Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients
Authors: M Diani, S Perego, V Sansoni, L Bertino, M Gomarasca, M Faraldi, PDM Pigatto, G Damiani, G Banfi, G Altomare, G Lombardi
Int J Mol Sci, 2019-11-10;20(22):.
Species: Human
Sample Types: Serum
Response of Sclerostin and Bone Turnover Markers to High Intensity Interval Exercise in Young Women: Does Impact Matter?
Authors: R Kouvelioti, N Kurgan, B Falk, WE Ward, AR Josse, P Klentrou
Biomed Res Int, 2018-11-01;2018(0):4864952.
Species: Human
Sample Types: Serum
Negative Association Between Sclerostin and INSL3 in Isolated Human Osteocytes and in Klinefelter Syndrome: New Hints for Testis-Bone Crosstalk
Authors: A Di Nisio, L De Toni, MS Rocca, M Ghezzi, R Selice, G Taglialavo, A Ferlin, C Foresta
J. Clin. Endocrinol. Metab., 2018-05-01;0(0):.
Species: Human
Sample Types: Serum
Wnt Signaling-Related Osteokines at Rest and Following Plyometric Exercise in Prepubertal and Early Pubertal Boys and Girls
Authors: P Klentrou, K Angrish, N Awadia, N Kurgan, R Kouvelioti, B Falk
Pediatr Exerc Sci, 2018-04-23;0(0):1-9.
Species: Human
Sample Types: Serum

Correlation of blood bone turnover biomarkers and Wnt signaling antagonists with AS, DISH, OPLL, and OYL
Authors: CC Niu, SS Lin, LJ Yuan, LH Chen, CY Yang, AN Chung, ML Lu, TT Tsai, PL Lai, WJ Chen
BMC Musculoskelet Disord, 2017-02-02;18(1):61.
Species: Human
Sample Types: Serum

Hormonal and systemic regulation of sclerostin
Authors: Matthew T Drake
Bone, 2016-12-10;0(0):.
Species: Human
Sample Types: Serum