Human SOX10 Antibody

Catalog #: MAB28642 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB28642-100
MAB28642-SP

Detection of SOX10 in U-2 OS Human Cell Line by Flow Cytometry.

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All SOX10 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SOX10 in direct ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2447A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human SOX10
Met1-Ala118
Accession # P56693

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of SOX10 antibody in U-2 OS Human Cell Line antibody by Flow Cytometry.

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Detection of SOX10 in U-2 OS Human Cell Line by Flow Cytometry. U-2 OS human osteosarcoma cell line was stained with Rabbit Anti-Human SOX10 Monoclonal Antibody (Catalog # MAB28642, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX10

SOX10 belongs to the SOX family of transcription factors which display diverse roles during development. In the central nervous system, SOX10 is required for the terminal differentiation of oligodendrocytes and myelination. In the peripheral nervous system, SOX10 maintains pluripotency of neural crest stem cells and suppresses neuronal differentiation.

References

Lo, L. et al. (2002) Development 129:1553.

Long Name

Transcription Factor SOX10

Entrez Gene IDs

6663 (Human)

Alternate Names

DOM; MGC15649; PCWH; SOX10; SRY (sex determining region Y)-box 10; SRY-related HMG-box gene 10; transcription factor SOX-10; WS4; WS4C; WS4mouse, human homolog of