Human SOX10 Antibody

SOX10 in SK‑Mel‑28 Human Cell Line. SOX10 was detected in immersion fixed SK‑Mel‑28 human malignant melanoma cell line using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

SOX10 in BG01V Human Embryonic Stem Cells. SOX10 was detected in immersion fixed BG01V human embryonic stem cells differentiated to neural crest stem cells using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

SOX10 in Human Melanoma Tissue. SOX10 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human SOX10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2864) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Mouse SOX10 by Immunohistochemistry PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and nuclear atypia (b), perineuronal satellitosis (c; N, neuronal nuclei), perivascular growth (d), intrafascicular growth (e), subarachnoid spreading (f), and areas of incipient necrosis (g; arrows point to pyknotic nuclei). (h–k) IF labeling of brain tumor sections for cell type specific markers. Nuclei labeled with DAPI are shown in blue. Tumor cells with high PDGFR alpha expression were highly proliferative, as seen by proliferation marker Ki67 (h), and express the OPC cell lineage markers Olig2, Sox2, Sox10, and Ng2, as well as the neural stem cell marker Nestin (i–k). Tumor cells were negative for immunosignal of astroglial marker GFAP, mature oligodendrocyte marker APC-CC1, and neuronal marker NeuN (l–n). Scale bars: 10 μm (a–g), 20 μm (h–n). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse SOX10 by Immunocytochemistry/Immunofluorescence Adult dynamin 2-depleted Schwann cells are replaced by dynamin 2-positive Schwann cells in remyelinated nerves.(A) Immunostainings for DNM2, SOX10 (SCs) and YFP (reporter-recombined SCs) on SN cross-sections of control* (MpzCreERT2:Rosa26-stoploxP/loxP-YFP) and P0ERT2-Dnm2KO* (MpzCreERT2:Dnm2loxP/loxP: Rosa26-stoploxP/loxP-YFP) mice at 4 wpT and 14 wpT. White arrows: YFP-recombined SCs that express DNM2 (in controls at 4 wpt and 14 wpt); black arrowheads: YFP-recombined SCs lacking DNM2 (in mutants at 4 wpt); white arrowheads: Non-recombined SCs expressing DNM2 (in controls and mutants at 4 wpt and 14 wpt). Scale bar = 25 μm for entire panel. (B) Quantification related to (A). Percentage of YFP+ cells among SOX10+ SCs/SN cross-sections at 4 wpT, 6 wpT, and 14 wpT. N = 3 mice/genotype, Two-Way ANOVA with Sidak’s multiple comparisons test. (C) Western blot analysis of cleaved caspase 3 (cC3) in SN lysates of control* and P0ERT2-Dnm2KO* mice at 4 wpT, 6 wpT and 14 wpT. Full-length blots in Supplementary file 1F. (D) Quantification referring to (C). Control average was set to 1. N = 3 mice/time point and genotype, Two-Way ANOVA with Sidak’s multiple comparisons test. Results in graphs represent means ±s.e.m.; ***p<0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30648534), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse SOX10 by Immunocytochemistry/Immunofluorescence Schwann cells lacking dynamin 2 show impaired cell cycle progression, reduced mitosis rate, and cytokinesis defects.(A) Schematic representation of the cell cycle phases marked by Ki-67 and EdU. (B) EdU-labeling, combined with immunostainings for Ki-67 and SOX10 on control and P0-Dnm2KO SN cross-sections at P5. Arrows: EdU+ Ki-67+ SOX10+ SCs, arrowheads: Ki-67+ SOX10+ SCs. Scale bar = 25 μm for entire panel. (C) Quantification of (B). Percentage of EdU+ SCs/SN cross-sections. N = 5 mice/time point and genotype, Two-Way ANOVA with Sidak’s multiple comparisons test. (D) Quantification of (B). Percentage of Ki-67+ SCs/SN cross-sections at P5. N = 5 mice/genotype, two-tailed unpaired Student´s t-test. (E) Quantification of (B). Percentage of EdU+ among Ki-67+ SCs/SN cross-section at P5. N = 5 mice/genotype, two-tailed unpaired Student´s t-test. (F) Quantification of mitotic events in cultured mouse SCs isolated from MpzCre:Rosa26-stoploxP/loxP-YFP (control*) and MpzCre:Dnm2loxP/loxP:Rosa26-stoploxP/loxP-YFP (P0-Dnm2KO*) SNs at P1, monitored by time-lapse microscopy for 24 hr. Each data point represents one individual animal (at least 42 cells/animal analyzed). N = 5 controls and seven mutant mice, two-tailed unpaired Student´s t-test. (G) Quantification of mitosis duration (minutes) of SCs of control* and P0-Dnm2KO* mice, monitored by time-lapse microscopy for 24 hr. Each data point represents one cell derived from a total of 5 control and seven mutant mice. Cells derived from each animal were isolated and analyzed separately, but pooled in one graph; two-tailed unpaired Student´s t-test. (H) Exemplary picture of a multinucleated Dnm2KO SCs after 48 hr in culture. Scale bar = 25 μm. (I) Quantification of multinucleated cells in control and Dnm2KO SCs after 24 hr, 48 hr and 76 hr in culture. N = 6 mice/genotype for 48 hr; N = 6 control* and n = 5 P0-Dnm2KO* mice for 24 hr and 72 hr, two-Way ANOVA with Sidak’s multiple comparisons test. (J) Exemplary time-lapse images of the mitosis of control and Dnm2KO SCs (YFP+ cells). Arrowheads: cell body of dividing SCs, black arrows: cytokinesis site. (K) Representative time-lapse images of Dnm2KO SC (YFP+) failing cytokinesis. The SC is undergoing mitosis, but fails to divide (arrowheads point to the cell body). Results in graphs represent means ±s.e.m.; *p<0.05, ***p<0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30648534), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse SOX10 by Immunohistochemistry PDGFR alpha driven brain tumors display features of high grade glioma.(a–g) Histopathological analysis of tumor areas by H&E staining shows a high concentration of mitotic figures (a, arrows), high cellularity and nuclear atypia (b), perineuronal satellitosis (c; N, neuronal nuclei), perivascular growth (d), intrafascicular growth (e), subarachnoid spreading (f), and areas of incipient necrosis (g; arrows point to pyknotic nuclei). (h–k) IF labeling of brain tumor sections for cell type specific markers. Nuclei labeled with DAPI are shown in blue. Tumor cells with high PDGFR alpha expression were highly proliferative, as seen by proliferation marker Ki67 (h), and express the OPC cell lineage markers Olig2, Sox2, Sox10, and Ng2, as well as the neural stem cell marker Nestin (i–k). Tumor cells were negative for immunosignal of astroglial marker GFAP, mature oligodendrocyte marker APC-CC1, and neuronal marker NeuN (l–n). Scale bars: 10 μm (a–g), 20 μm (h–n). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25683249), licensed under a CC-BY license. Not internally tested by R&D Systems.