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Human SOX21 Antibody

Catalog #: AF3538
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3538
AF3538-SP
SOX21 in NTera‑2 Human Cell Line.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX21 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SOX21 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX1, rhSOX2 and rhSOX15 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SOX21
Ser2-Leu276 (Gly102Ser)
Accession # Q9Y651
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human SOX21
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human glioblastoma
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry SOX21 antibody in NTera-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

SOX21 in NTera‑2 Human Cell Line. SOX21 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Goat Anti-Human SOX21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3538) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry View Larger

Detection of SOX21 in Human Glioblastoma. SOX21 was detected in immersion fixed paraffin-embedded sections of human glioblastoma using Goat Anti-Human SOX21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3538) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017) or the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX21

SOX21 is a member of the B group of the SOX family HMG-box transcription factors. It is a 276 amino acid residue protein that has an N-terminal HMG-box and a C‑terminal domain that is required for the SOX21 neurogenesis function. Human and mouse SOX 21 share 99% amino acid sequence identity.

Long Name
Transcription Factor SOX21
Entrez Gene IDs
11166 (Human); 223227 (Mouse); 306168 (Rat)
Alternate Names
SOX21; SOX25; SOX25SOX-A; SOXA; SRY (sex determining region Y)-box 21; SRY-box 21; transcription factor SOX-21

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Citations for Human SOX21 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Sox21 promotes hippocampal adult neurogenesis via the transcriptional repression of the Hes5 gene
    Authors: Satoru Matsuda, Ken-ichiro Kuwako, Hirotaka James Okano, Shuichi Tsutsumi, Hiroyuki Aburatani, Yumiko Saga et al.
    The Journal of Neuroscience
  2. SOX21 modulates SOX2-initiated differentiation of epithelial cells in the extrapulmonary airways
    Authors: Evelien Eenjes, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Gabriela G Edel, Floor Benthem, Lisette de Kreij-de Bruin et al.
    eLife
  3. Distinct roles for SOX2 and SOX21 in differentiation, distribution and maturation of pulmonary neuroendocrine cells
    Authors: Evelien Eenjes, Floor Benthem, Anne Boerema-de Munck, Marjon Buscop-van Kempen, Dick Tibboel, Robbert J. Rottier
    Cellular and Molecular Life Sciences
  4. Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
    Authors: N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay
    Cell Rep, 2020-05-05;31(5):107599.
    Species: Human
    Sample Types:
    Applications: IF
  5. Characterization of the neural stem cell gene regulatory network identifies OLIG2 as a multifunctional regulator of self-renewal.
    Authors: Mateo J, van den Berg D, Haeussler M, Drechsel D, Gaber Z, Castro D, Robson P, Crawford G, Flicek P, Ettwiller L, Wittbrodt J, Guillemot F, Martynoga B
    Genome Res, 2014-10-07;25(1):41-56.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  6. Sox21 deletion in mice causes postnatal growth deficiency without physiological disruption of hypothalamic-pituitary endocrine axes
    Authors: Leonard Y. M. Cheung, Hideyuki Okano, Sally A. Camper
    Molecular and Cellular Endocrinology

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