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Human SP-D QuicKit ELISA

Catalog #: QK1920 Datasheet / COA / SDS
Human SP-D QuicKit ELISA
Catalog # Availability Size / Price Qty
QK1920
SK1920
PK1920
Control Products Available
QC320: QuicKit Immunoassay Control for Human SP-D
Human SP-D Quantikine QuicKit ELISA Standard Curve
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human SP-D QuicKit ELISA Summary

Assay Length
80 minutes
Specificity
Natural and recombinant human SP-D.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine QuicKit Human SP-D Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human SP-D levels in cell culture supernates, serum, plasma, and BALF. It contains recombinant human SP-D and antibodies raised against the recombinant protein. Results obtained for naturally occurring human SP-D showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural SP-D.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least four technicians

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 20 20 10 10
Mean (ng/mL) 4.65 23.8 4.37 22
Standard Deviation 0.21 1.27 0.277 1.62
CV% 4.5 5.3 6.3 7.4

Recovery

The recovery of human SP-D spiked to three different levels in samples throughout the range of the assay in cell culture media was evaluated.

Sample Type Average % Recovery Range %
Bronchoalveolar Lavage Fluid (n=2) 96 93-99
Cell Culture Media (n=4) 110 106-114
EDTA Plasma (n=2) 90 81-99
Heparin Plasma (n=2) 86 79-95
Serum (n=2) 90 77-100

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human SP-D were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. 
Human SP-D Quantikine QuicKit ELISA Standard Curve

Scientific Data

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Human SP-D Quantikine QuicKit ELISA Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SP-D

Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.

Long Name:
Surfactant Pulmonary Associated Protein D
Entrez Gene IDs:
6441 (Human); 20390 (Mouse)
Alternate Names:
COLEC7collectin-7; Collectin 7; Collectin-7; Lung surfactant protein D; PSPD; PSP-D; SFTP4; SFTP4pulmonary surfactant-associated protein D; SFTPD; SPD; SP-D; SP-Dpulmonary surfactant apoprotein; surfactant protein D; surfactant, pulmonary-associated protein D; surfactant-associated protein, pulmonary 4

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