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Human Sp3 Antibody

Catalog #: AF4256 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4256
AF4256-SP
Detection of Human Sp3 by Western Blot.
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Human Sp3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Sp3 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Sp3
Asp53-Ser309
Accession # Q02447
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Sp3 antibody by Western Blot. View Larger

Detection of Human Sp3 by Western Blot. Western blot shows nuclear extracts of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and, Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Sp3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4256) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Sp3 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sp3

Sp3 is a member of the Sp1 family of C2H2-zinc-finger transcription factors. Sp3 binds to GT and GC boxes in promoters and can either stimulate or repress transcription. Knock out mice indicate that Sp3 is important particularly in erythroid and myeloid cell lineages.

Long Name
Sp3 Transcription Factor
Entrez Gene IDs
6670 (Human); 20687 (Mouse); 367846 (Rat)
Alternate Names
DKFZp686O1631; GC-binding transcription factor Sp3; Sp3 transcription factor; Sp3; specificity protein 3; SPR2; SPR-2; transcription factor Sp3

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