Human Spinesin Antibody Summary
Tyr27-Leu413
Accession # Q0P514
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Spinesin in Human brain (spinal cord). Spinesin was detected in immersion fixed paraffin-embedded sections of Human brain (spinal cord) using Goat Anti-Human Spinesin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2495) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to motoneurons in ventral horn in the spinal cord.
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Spinesin in Human brain. Spinesin was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human Spinesin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2495) at 1.5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (red; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Spinesin
Spinesin, encoded by the TMPRSS5 gene, is a member of type II transmembrane serine proteases (TTSPs) (1). Human Spinesin contains the following structural domains: a short N-terminal cytoplasmic tail (amino acid residues 1‑49), a transmembrane domain (residues 50‑70), a stem region and a serine protease domain (residues 71‑457) (2). The domain structure of Spinesin is common to other TTSPs, many of which have additional domains. The stem region of Spinesin contains a scavenger receptor-like domain. The ectodomain of human Spinesin (residues 71‑457) was expressed and purified as a single chain pro-enzyme. The deduced amino acid sequence contains a Leu instead of a Phe residue at position 369; the former is identical to the mouse protein (3, 4). The pro-enzyme can be activated and the resulting enzyme activity can be measured as described in the Activity Assay Protocol.
- Hooper, J.D. et al. (2001) J. Biol. Chem. 276:857.
- Yamaguchi, Y. et al. (2002) J. Biol. Chem. 277:6806.
- Carninci, P. et al. (2000) Genome Res. 10:1617.
- Shibata, K. et al. (2000) Genome Res. 10:1757.
Product Datasheets
Citation for Human Spinesin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Transmembrane protease serine 5: a novel Schwann cell plasma marker for CMT1A
Authors: Hongge Wang, Matthew Davison, Kathryn Wang, Tai‐He Xia, Martin Kramer, Katherine Call et al.
Annals of Clinical and Translational Neurology
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