Human SREBP2 Antibody Summary
Leu242-Asp450
Accession # Q12772
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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SREBP2 in HepG2 Human Cell Line. SREBP2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human SREBP2 Monoclonal Antibody (Catalog # MAB7119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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SREBP2 in Human Kidney. SREBP2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human SREBP2 Monoclonal Antibody (Catalog # MAB7119) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SREBP2
SREBP2 (Sterol Regulatory Element-Binding Protein 2; also bHLHD2 and SREBF2) is a 120-125 kDa member of the SREBP family of proteins. It is ubiquitously expressed and found in the intracellular membrane fraction of cells. SREBP2 is a transcriptional factor initially embedded in the ER as an inactive precursor associated with SCAP. When necessary, SCAP mediates SREBP2 transfer to the Golgi, where two resident proteases remove the N-terminus from SREBP2, and the N-terminus is transported into the nucleus. Here, SREBP2 acts as a transcription factor, activating the LDLR and cholesterol synthesis genes. The human SREBP2 precursor is an 1141 amino acid (aa) two transmembrane protein whose N- and C-termini are cytoplasmic. The two cytoplasmic domains span aa 1-479 and 555-1141, respectively. Proteolytic cleavage between Leu484-Cys485 generates the 64-66 kDa SREBP2 transcription factor. This fragment contains a bHLH DNA binding domain (aa 330-380) and one Leu zipper region (aa 381-401). Homodimerization of SREBP2 is necessary for nuclear translocation. There is one potential isoform that shows a deletion of aa 274-276 coupled to a 96 aa substitution for aa 580-1141. A second isoform (known in rodent) shows a premature truncation after Val463 and runs at 55 kDa in SDS-PAGE. Over aa 242-450, human SREBP2 shares 97% aa identity with mouse SREBP2.
Product Datasheets
Citations for Human SREBP2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
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Lysosomal integral membrane protein-2 (LIMP-2/SCARB2) is involved in lysosomal cholesterol export
Authors: Heybrock S, Kanerva K, Meng Y et al.
Nat Commun
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A Chemokine Regulatory Loop Induces Cholesterol Synthesis in Lung-Colonizing Triple-Negative Breast Cancer Cells to Fuel Metastatic Growth
Authors: Han B, Alonso-Valenteen F, Wang Z Et Al.
Molecular therapy : the journal of the American Society of Gene Therapy
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Palmitate-Induced SREBP1 Expression and Activation Underlies the Increased BACE 1 Activity and Amyloid Beta Genesis
Authors: Gurdeep Marwarha, Kate Claycombe-Larson, Jonah Lund, Othman Ghribi
Molecular Neurobiology
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Ferredoxin 1 is essential for embryonic development and lipid homeostasis
Authors: Mohibi, S;Zhang, Y;Perng, V;Chen, M;Zhang, J;Chen, X;
eLife
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Investigating the Diagnostic and Therapeutic Potential of SREBF2-Related Lipid Metabolism Genes in Colon Cancer
Authors: Ma, Y;Wang, Z;Sun, J;Tang, J;Zhou, J;Dong, M;
OncoTargets and therapy
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
ARMC5-CUL3 E3 ligase targets full-length SREBF in adrenocortical tumor
Authors: Y Okuno, A Fukuhara, M Otsuki, I Shimomura
JCI Insight, 2022-08-22;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Eicosapentaenoic acid in combination with EPHA2 inhibition shows efficacy in preclinical models of triple-negative breast cancer by disrupting cellular cholesterol efflux
Authors: AM Torres-Ado, H Vitrac, Y Qi, L Tan, KR Levental, YY Fan, P Yang, RS Chapkin, BL Eckhardt, NT Ueno
Oncogene, 2018-11-20;0(0):.
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