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Human SRPK3 Antibody

Catalog #: MAB7230
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7230
MAB7230-SP
Detection of Human SRPK3 by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SRPK3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SRPK3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) SRPK1 or rhSRPK2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 722534
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human SRPK3
Arg247-Ser316
Accession # Q9UPE1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human SRPK3 antibody by Western Blot. View Larger

Detection of Human SRPK3 by Western Blot. Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SRPK3 Monoclonal Antibody (Catalog # MAB7230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SRPK3 at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SRPK3

SRPK3 (Ser/Arg-rich protein specific kinase 3), also called MSSK-1 (muscle-specific serine kinase 1) or STK23 (Ser/Thr kinase 23) is a 59 kDa (predicted) cytoplasmic member of the CMGC Ser/Thr protein kinase family. SRPK3 is principally expressed in heart and skeletal muscle. SRPKs selectively phosphorylate Ser on RS domain-containing proteins, initiating mRNA splicing and maturation.  The 533 amino acid (aa) human SRPK3 contains a split kinase domain (aa 79‑292 and 347‑531) separated by a hinge region. A 491 aa isoform shows an alternate translation start site at aa 43. Within the region used as an immunogen, human SRPK3 shares 87% and 86% aa identity with mouse and rat SRPK3, respectively.

Long Name
Ser/Arg-rich Protein-specific Kinase 3
Entrez Gene IDs
26576 (Human); 56504 (Mouse); 293854 (Rat)
Alternate Names
MGC102944; MSSK1; MSSK-1; MSSK1EC 2.7.11.1; Muscle-specific serine kinase 1; serine arginine rich protein-specific kinase 3; Serine/arginine-rich protein-specific kinase 3; serine/threonine kinase 23; Serine/threonine-protein kinase 23; serine/threonine-protein kinase SRPK3; SFRS protein kinase 3; SRPK3; SR-protein-specific kinase 3; SRSF protein kinase 3; STK23

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Citation for Human SRPK3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Functional Diversification of SRSF Protein Kinase to Control Ubiquitin-Dependent Neurodevelopmental Signaling
    Authors: Bustos F, Segarra-Fas A, Nardocci G et al.
    Dev Cell

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