Human SSBP1 Antibody Summary
Met1-Glu148
Accession # Q04837
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human SSBP1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, MOLT-4 human acute lymphoblastic leukemia cell line, K562 human chronic myelogenous leukemia cell line, and UT-7 human acute myeloid leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SSBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6588) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SSBP1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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SSBP1 in HL‑60 Human Cell Line. SSBP1 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Sheep Anti-Human SSBP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6588) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SSBP1
SSBP1 (Single-Stranded DNA –Binding Protein; also MtSSB and PWP1-interacting protein 17) is a 17-18 kDa member of the mtSSB protein family. SSBP1 should not be confused with SSB1/SOSS-B1. Although SSBP1 binds single-stranded mitochondrial DNA, it is not the product of a mitochondrial gene. Rather, it is synthesized on ribosomes in the cytosol, and undergoes transport into mitochondria via an N-terminal mitochondrial localization signal. After import, the localization motif is cleaved, allowing for homotetramerization. Upon DNA replication, SSBP1 is phosphorylated by CDK, allowing for DNA duplication. Human SSBP1 is 148 amino acids (aa) in length. It contains a cleavable mitochondrial targeting sequence (aa 1-16), an SSB domain (aa 30-141), one phosphorylation (Ser79) and one acetylation site (Lys113). There is one potential isoform that shows a 25 aa substitution for aa 135-148. Full-length human SSBP1 shares 92% aa identity with mouse SSBP1.
Product Datasheets
Citations for Human SSBP1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy.
Authors: Katharina Z, Anna L, Nicolas N et al.
Mol Cell Proteomics.
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Dominant mutations in mtDNA maintenance gene SSBP1 cause optic atrophy and foveopathy
Authors: C Piro-Mégy, E Sarzi, A Tarrés-Sol, M Péquignot, F Hensen, M Quilès, G Manes, A Chakrabort, A Sénéchal, B Bocquet, C Cazevieill, A Roubertie, A Müller, M Charif, D Goudenège, G Lenaers, H Wilhelm, U Kellner, N Weisschuh, B Wissinger, X Zanlonghi, C Hamel, JN Spelbrink, M Solà, C Delettre
J. Clin. Invest., 2020-01-02;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Temporal Analysis of Protein Ubiquitylation and Phosphorylation During Parkin-Dependent Mitophagy.
Authors: Katharina Z, Anna L, Nicolas N et al.
Mol Cell Proteomics.
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