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Human ST2/IL-33R ELISA Kit - Quantikine

Catalog #: DST200
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human ST2/IL-33R Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
13.5 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human ST2 as well as free ST2 and IL-33 complexed ST2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human ST2 in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (ng/mL)Range (ng/mL)Standard Devitation (ng/mL)
Serum (n=35)13.06.74-20.44.15
Heparin plasma (n=35)12.35.72-19.84.10
EDTA plasma (n=35)12.24.90-19.94.08

Cell Culture Supernates: BJAB human Burkitt's lymphoma cells (1 x 105 cells/mL) were seeded in DMEM supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human ST2, and measured 126 pg/mL.

BUD-8 human fibroblast cells were seeded in MEM NEAA 90% supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate until confluent. An aliquot of the cell culture supernate was removed, assayed for human ST2, and measured 273 pg/mL.

U-87 MG human glioblastoma/astrocytoma cells (2.1 x 105 cells/mL) were seeded in MEM supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 2 mM L-glutamine until confluent. An aliquot of the cell culture supernate was removed, assayed for human ST2, and measured 63.9 pg/mL.

HUVEC human umbilical vein endothelial cells (1 x 104 cells/mL) were seeded in EGM-2 media until confluent. An aliquot of the cell culture supernate was removed, assayed for human ST2, and measured 29,920 pg/mL. 

HUT-78 human cutaneous T cell lymphoma cells (1 x 104 cells/mL) were seeded in RPMI supplemented with 10% fetal bovine serum, 5 μM beta -mercaptoethanol, and 10 ng/mL recombinant human IL-2 until confluent. An aliquot of the cell culture supernate was removed, assayed for human ST2, and measured 54.9 pg/mL. 

Product Summary

The Quantikine Human ST2/IL-33 R Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ST2 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human ST2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ST2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring ST2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 273 628 1027 262 642 1064
Standard Deviation 15.2 27.5 46.3 18.7 34.7 67.2
CV% 5.6 4.4 4.5 7.1 5.4 6.3

Recovery

The recovery of ST2 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 89-106

Linearity

To assess the linearity of the assay, samples containing or spiked with high concentrations of ST2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human ST2/IL-1 R4 ELISA Linearity

Scientific Data

N/A Human ST2/IL-1 R4 ELISA Standard Curve View Larger

Human ST2/IL-1 R4 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ST2/IL-33R

ST2, also known as IL-1 R4, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.

Long Name:
Interleukin 33 Receptor
Entrez Gene IDs:
9173 (Human); 17082 (Mouse)
Alternate Names:
DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product datasheet for complete assay procedure.
 
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.

Prepare all reagents, standard dilutions, and samples as directed in the product insert.

Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

Add 50 µL of Assay Diluent to each well.

add diluent to well graphic

Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.

add diluent to well graphic

Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.

add diluent to well graphic

Aspirate and wash 4 times.

Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

add substrate to well graphic

Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

add stop solution to well graphic

Citations for Human ST2/IL-33R Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

38 Citations: Showing 1 - 10
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  1. Endothelial CD34 expression and regulation of immune cell response in-vitro
    Authors: Arakelian, L;Lion, J;Churlaud, G;Bargui, R;Thierry, B;Mutabazi, E;Bruneval, P;Alberdi, AJ;Doliger, C;Veyssiere, M;Larghero, J;Mooney, N;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Evaluating Cardiotoxicity in Breast Cancer Patients Treated with HER2 Inhibitors: Could a Combination of Radionuclide Ventriculography and Cardiac Biomarkers Predict the Cardiac Impact?
    Authors: M Gherghe, AM Lazar, MD Mutuleanu, CI Bordea, S Ionescu, RI Mihaila, C Petroiu, AE Stanciu
    Cancers, 2022-12-29;15(1):.
    Species: Human
    Sample Types: Serum
  3. Blood-biomarkers and devices for atrial fibrillation screening: Lessons learned from the AFRICAT (Atrial Fibrillation Research In CATalonia) study
    Authors: E Palà, A Bustamante, JL Clúa-Espun, J Acosta, F González-L, SD Santos, D Ribas-Segu, J Ballesta-O, A Penalba, M Giralt, I Lechuga-Du, D Gentille-L, A Pedrote, MÁ Muñoz, J Montaner
    PLoS ONE, 2022-08-23;17(8):e0273571.
    Species: Human
    Sample Types: Serum
  4. The circulating proteomic signature of alcohol-associated liver disease
    Authors: J Luther, AG Vannier, EA Schaefer, RP Goodman
    JCI Insight, 2022-07-22;7(14):.
    Species: Human
    Sample Types: Serum
  5. Effects of liraglutide vs. lifestyle changes on soluble suppression of tumorigenesis-2 (sST2) and galectin-3 in obese subjects with prediabetes or type 2 diabetes after comparable weight loss
    Authors: P Simeone, R Tripaldi, A Michelsen, T Ueland, R Liani, S Ciotti, KI Birkeland, HL Gulseth, A Di Casteln, F Cipollone, P Aukrust, A Consoli, B Halvorsen, F Santilli
    Cardiovascular Diabetology, 2022-03-11;21(1):36.
    Species: Human
    Sample Types: Serum
  6. Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development
    Authors: E Mai, J Chan, L Goon, BK Ego, J Bevers, T Wong, M Wong, R Corpuz, H Xi, J Wu, K Schneider, D Seshasayee, M Grimbaldes, G Nakamura, VB Indjeian, M van Looker, KM Loyet, L Comps-Agra
    Journal of Translational Medicine, 2021-12-20;19(1):517.
    Species: Human
    Sample Types: Vitreous Humor
  7. Circulating miR-19b-3p as a Novel Prognostic Biomarker for Acute Heart Failure
    Authors: Y Su, Y Sun, Y Tang, H Li, X Wang, X Pan, W Liu, X Zhang, F Zhang, Y Xu, C Yan, SB Ong, D Xu
    Journal of the American Heart Association, 2021-10-06;10(20):e022304.
    Species: Human
    Sample Types: Serum
  8. Randomised controlled trial into the role of ramipril in fibrosis reduction in rheumatic heart disease: the RamiRHeD trial protocol
    Authors: AM Ambari, B Setianto, A Santoso, B Radi, B Dwiputra, E Susilowati, F Tulrahmi, A Wind, MJM Cramer, P Doevendans
    BMJ Open, 2021-09-13;11(9):e048016.
    Species: Human
    Sample Types: Plasma
  9. Myocardial fibrosis combined with NT-proBNP improves the accuracy of survival prediction in ADHF patients
    Authors: Y Yao, L Feng, Y Sun, S Wang, J Sun, B Hu
    BMC cardiovascular disorders, 2021-05-28;21(1):264.
    Species: Human
    Sample Types: Serum
  10. Analysis of the Plasma Metabolome after Trauma, Novel Circulating Sphingolipid Signatures, and In-Hospital Outcomes
    Authors: A Cyr, Y Zhong, SE Reis, RA Namas, A Amoscato, B Zuckerbrau, J Sperry, R Zamora, Y Vodovotz, TR Billiar
    Journal of the American College of Surgeons, 2021-01-14;0(0):.
    Species: Human
    Sample Types: Plasma
  11. Vascular density with optical coherence tomography angiography and systemic biomarkers in low and high cardiovascular risk patients
    Authors: MA Hannappe, L Arnould, A Méloux, B Mouhat, F Bichat, M Zeller, Y Cottin, C Binquet, C Vergely, C Creuzot-Ga, C Guenancia
    Sci Rep, 2020-10-07;10(1):16718.
    Species: Human
    Sample Types: Serum
  12. Systemic right ventricle in elderly patients with congenitally corrected transposition of the great arteries: Clinical profile, cardiac biomarkers, and echocardiographic parameters
    Authors: E Kowalik, A Kwiatek-Wr, A Klisiewicz, A Lutynska, EK Biernacka, M Kowalski, P Hoffman
    Anatol J Cardiol, 2020-08-01;24(2):92-96.
    Species: Human
    Sample Types: Plasma
  13. IL-33 and its decoy sST2 in patients with Alzheimer's disease and mild cognitive impairment
    Authors: M Saresella, I Marventano, F Piancone, F La Rosa, D Galimberti, C Fenoglio, E Scarpini, M Clerici
    J Neuroinflammation, 2020-06-06;17(1):174.
    Species: Human
    Sample Types: Serum
  14. Immune Profiling To Predict Outcome of Clostridioides difficile Infection
    Authors: MM Abhyankar, JZ Ma, KW Scully, AJ Nafziger, AL Frisbee, MM Saleh, GR Madden, AR Hays, M Poulter, WA Petri
    MBio, 2020-05-26;11(3):.
    Species: Human
    Sample Types:
  15. Alteration of the IL-33-sST2 pathway in hypertensive patients and a mouse model
    Authors: X Yin, H Cao, Y Wei, HH Li
    Hypertens. Res., 2019-06-24;0(0):.
    Species: Human
    Sample Types: Serum
  16. Systemic Inflammation Precedes Microalbuminuria in Diabetes
    Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk
    Kidney Int Rep, 2019-06-21;4(10):1373-1386.
    Species: Human
    Sample Types: Serum
  17. IL-33 drives group 2 innate lymphoid cell-mediated protection during Clostridium difficile infection
    Authors: AL Frisbee, MM Saleh, MK Young, JL Leslie, ME Simpson, MM Abhyankar, CA Cowardin, JZ Ma, P Pramoonjag, SD Turner, AP Liou, EL Buonomo, WA Petri
    Nat Commun, 2019-06-20;10(1):2712.
    Species: Human
    Sample Types: Serum
  18. Computational evidence for an early, amplified systemic inflammation program in polytrauma patients with severe extremity injuries
    Authors: K Almahmoud, A Abboud, RA Namas, R Zamora, J Sperry, AB Peitzman, MS Truitt, GE Gaski, TO McKinley, TR Billiar, Y Vodovotz
    PLoS ONE, 2019-06-04;14(6):e0217577.
    Species: Human
    Sample Types: Plasma
  19. Serum soluble ST2 as a marker of renal scar in pediatric upper urinary tract infection
    Authors: N Ohta, H Yasudo, M Mizutani, T Matsushige, R Fukano, S Kittaka, K Maehara, K Ichihara, S Ohga, S Hasegawa
    Cytokine, 2019-05-29;120(0):258-263.
    Species: Human
    Sample Types: Serum
  20. Combination of Plasma Biomarkers and Clinical Data for the Detection of Myocardial Fibrosis or Aggravation of Heart Failure Symptoms in Heart Failure with Preserved Ejection Fraction Patients
    Authors: CK Wu, MM Su, YF Wu, JJ Hwang, LY Lin
    J Clin Med, 2018-11-08;7(11):.
    Species: Human
    Sample Types: Plasma
  21. Biomarkers as predictors of sudden cardiac death in coronary artery disease patients with preserved left ventricular function (ARTEMIS study)
    Authors: ES Lepojärvi, HV Huikuri, OP Piira, AM Kiviniemi, JA Miettinen, T Kenttä, O Ukkola, JS Perkiömäki, MP Tulppo, MJ Junttila
    PLoS ONE, 2018-09-18;13(9):e0203363.
    Species: Human
    Sample Types: Serum
  22. Serum sST2 levels predict severe exacerbation of asthma
    Authors: M Watanabe, K Nakamoto, T Inui, M Sada, K Honda, M Tamura, Y Ogawa, T Yokoyama, T Saraya, D Kurai, H Ishii, H Takizawa
    Respir. Res., 2018-09-03;19(1):169.
    Species: Human
    Sample Types: Serum
  23. Genetic variants in IL-33/ST2 pathway with the susceptibility to hepatocellular carcinoma in a Chinese population
    Authors: ZH Wei, YY Li, SQ Huang, ZQ Tan
    Cytokine, 2018-04-12;0(0):.
    Species: Human
    Sample Types: Plasma
  24. Genetic regulation ofIL1RL1methylation and IL1RL1-a protein levels in asthma
    Authors: FN Dijk, C Xu, E Melén, AE Carsin, A Kumar, IM Nolte, O Gruzieva, G Pershagen, NS Grotenboer, OEM Savenije, JM Antó, I Lavi, C Dobaño, J Bousquet, P van der Vl, RJP van der Va, JC de Jongste, MC Nawijn, S Guerra, DS Postma, GH Koppelman
    Eur. Respir. J., 2018-03-08;51(3):.
    Species: Human
    Sample Types: Serum
  25. The prognostic value of sST2 and galectin-3 considering different aetiologies in non-ischaemic heart failure
    Authors: D Binas, H Daniel, A Richter, V Ruppert, KD Schlüter, B Schieffer, S Pankuweit
    Open Heart, 2018-02-26;5(1):e000750.
    Species: Human
    Sample Types: Serum
  26. Interleukin-33 modulates inflammation in endometriosis
    Authors: JE Miller, SP Monsanto, SH Ahn, K Khalaj, AT Fazleabas, SL Young, BA Lessey, M Koti, C Tayade
    Sci Rep, 2017-12-20;7(1):17903.
    Species: Human
    Sample Types: Plasma
  27. Long-term systolic function in children and young adults after hematopoietic stem cell transplant
    Authors: SJ Rotz, CE Dandoy, MD Taylor, S Jodele, JL Jefferies, A Lane, JA El-Bietar, AW Powell, SM Davies, TD Ryan
    Bone Marrow Transplant., 2017-07-17;0(0):.
    Species: Human
    Sample Types: Plasma
  28. Plasma Level of Soluble ST2 in Chronically Infected HIV-1 Patients with Suppressed Viremia
    Authors: M Younas, C Psomas, V Mehraj, R Cezar, P Portales, E Tuaillon, A Guigues, J Reynes, P Corbeau, JP Routy
    Open AIDS J, 2017-04-26;11(0):32-35.
    Species: Human
    Sample Types: Plasma
  29. The injured heart: early cardiac effects of hematopoietic stem cell transplantation in children and young adults
    Authors: SJ Rotz, TD Ryan, S Jodele, JL Jefferies, A Lane, A Pate, R Hirsch, J Hlavaty, AE Levesque, MD Taylor, M Cash, KC Myers, JA El-Bietar, SM Davies, CE Dandoy
    Bone Marrow Transplant., 2017-04-10;0(0):.
    Species: Human
    Sample Types: Plasma
  30. Serum Galectin-3 and ST2 as predictors of unfavorable outcome in stable dilated cardiomyopathy patients
    Authors: C Wojciechow, E Romuk, E Nowalany-K, W Jache?
    Hellenic J Cardiol, 2017-03-28;0(0):.
    Species: Human
    Sample Types: Serum
  31. Targeting LOXL2 for cardiac interstitial fibrosis and heart failure treatment
    Nat Commun, 2016-12-14;7(0):13710.
    Species: Human
    Sample Types: Plasma
  32. Carotid plaque and bone density and microarchitecture in psoriatic arthritis: the correlation with soluble ST2
    Sci Rep, 2016-08-24;6(0):32116.
    Species: Human
    Sample Types: Plasma
  33. IL-33 ameliorates Alzheimer's disease-like pathology and cognitive decline
    Authors: AK Fu, KW Hung, MY Yuen, X Zhou, DS Mak, IC Chan, TH Cheung, B Zhang, WY Fu, FY Liew, NY Ip
    Proc Natl Acad Sci USA, 2016-04-18;0(0):.
    Species: Human
    Sample Types: Serum
  34. Reduced IL-33 plasma levels in aplastic anemia.
    Authors: Sun M, Ma H, Che Y, Cui X
    Cancer Cell Int, 2015-12-16;15(0):118.
    Species: Human
    Sample Types: Plasma
  35. Phenotypic and genotypic association of epithelial IL1RL1 to human TH2-like asthma.
    Authors: Traister R, Uvalle C, Hawkins G, Meyers D, Bleecker E, Wenzel S
    J Allergy Clin Immunol, 2014-08-01;135(1):92-9.
    Species: Human
    Sample Types: BALF
  36. IL1RL1 gene variants and nasopharyngeal IL1RL-a levels are associated with severe RSV bronchiolitis: a multicenter cohort study.
    Authors: Faber TE, Schuurhof A, Vonk A, Koppelman GH, Hennus MP, Kimpen JL, Janssen R, Bont LJ
    PLoS ONE, 2012-05-04;7(5):e34364.
    Species: Human
    Sample Types: Tissue Secretion
  37. High Levels of Soluble ST2 and Low Levels of IL-33 in Sera of Patients with HIV Infection.
    Authors: Miyagaki T, Sugaya M
    J. Invest. Dermatol., 2010-12-09;131(0):794.
    Species: Human
    Sample Types: Serum
  38. IL-33 mediates inflammatory responses in human lung tissue cells.
    Authors: Yagami A, Orihara K, Morita H
    J. Immunol., 2010-10-06;185(10):5743-50.
    Species: Human
    Sample Types: Cell Culture Supernates

FAQs

  1. Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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Human ST2/IL-33 R Quantikine ELISA Kit
By Ivana Marventano on 12/01/2016
Sample Tested: Human serum
Human ST2/IL-33 R Quantikine ELISA Kit DST200
Human ST2/IL-1 R4 Quantikine ELISA Kit
By Anonymous on 07/26/2016
Sample Tested: Cell culture supernatant,Cell Lysates,EDTA Plasma