Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

Catalog #: AF6905
1 Citation Datasheet / COA / SDS

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AF6905
AF6905-SP

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Detection of Human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1 by Western Blot.

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All ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human ST3GAL2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1
Accession # Q11201

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ST3 beta -Gal alpha -2,3-Sialyltransferase 1/ST3GAL1 by Western Blot. Western blot shows lysates of human placenta. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ST3 beta -Gal a-2,3-Sialyltransferase 1/ST3GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6905) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta -Gal a-2,3-Sialyltransferase 1/ ST3GAL1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1

ST3 beta -Gal alpha -2,3-Sialyltransferase 1 (ST3GAL1, also known as SIAT4 and SIAT4A) is a 45 kDa member of glycosyltransferase family 29 that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The full length ST3GAL1is a type II Golgi membrane glycoprotein that can be proteolytically processed to a soluble form. ST3Gal1 contributes to the alpha2-3-sialylation of O-glycans such as T antigens. Upon maturation of Dendritic cells derived from monocytes ST3GAl1 is altered in a stimulus-dependent manner. Multiple isoforms of human ST3GAL1 have been described. Human ST3GAL1 shares 85% identity with mouse ST3GAL1.

Long Name

ST3 beta-Galactoside alpha-2,3-Sialyltransferase 1

Entrez Gene IDs

6482 (Human); 20442 (Mouse); 362924 (Rat)

Alternate Names

Alpha 2,3-ST 1; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1; DKFZp666E036; DKFZp779K2051; EC 2.4.99; EC 2.4.99.4; Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase; Gal-NAc6S; sialyltransferase 4A (beta-galactosidase alpha-2,3-sialytransferase); sialyltransferase 4A (beta-galactoside alpha-2,3-sialyltransferase); sialyltransferase 4A (beta-galactoside alpha-2,3-sialytransferase); Sialyltransferase 4A; SIAT4; SIAT4A; SIAT4-A; SIATFL; SIATFLFLJ36548; ST3 beta-galactoside alpha-2,3-sialyltransferase 1; ST3Gal I; ST3GAL1; ST3GalA; ST3GalA.1MGC9183; ST3GalI; ST3GalIA; ST3GalIA1,Beta-galactoside alpha-2,3-sialyltransferase 1; ST3O; ST3OST3GalA

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer
Authors: Dashzeveg, NK;Jia, Y;Zhang, Y;Gerratana, L;Patel, P;Shajahan, A;Dandar, T;Ramos, EK;Almubarak, HF;Adorno-Cruz, V;Taftaf, R;Schuster, EJ;Scholten, D;Sokolowski, MT;Reduzzi, C;El-Shennawy, L;Hoffmann, AD;Manai, M;Zhang, Q;D'Amico, P;Azadi, P;Colley, KJ;Platanias, LC;Shah, AN;Gradishar, WJ;Cristofanilli, M;Muller, WA;Cobb, BA;Liu, H;
Cancer discovery
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

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