Human ST6 Gal Sialyltransferase 1/ST6GAL1 Biotinylated Antibody

Catalog #: BAF5924 Datasheet / COA / SDS

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BAF5924

All ST6 Gal Sialyltransferase 1/ST6GAL1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ST6 Gal Sialyltransferase 1/ST6GAL1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Gal Sialyltransferase 1/ST6GAL1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Gal Sialyltransferase 1/ST6GAL1
Lys27-Cys406
Accession # P15907

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ST6 Gal Sialyltransferase 1/ST6GAL1 (Catalog # 5924-GT)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST6 Gal Sialyltransferase 1/ST6GAL1

Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion (1). Sialyltransferases are key enzymes that regulate the cellular levels of sialic acid-containing molecules. Beta-galactosamide alpha‑2,6‑sialyltransferase 1 encoded by the ST6GAL1 gene is a type-II membrane protein localized in the trans-Golgi network and catalyzes 2,6-sialylation of Gal beta 1,4‑GlcNAc structures on N-glycans (2). The enzyme is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB‑6, CD75, and CD76 (3). ST6GAL1 is highly expressed in the liver and also expressed in most other tissues to some extent (4). ST6GAL1 deficiency causes abnormalities in B-cell immunoreactivity (5). The expression and activity of ST6GAL1 are associated with tumor metastasis in breast (6) and colon (7) cancers. The majority of ST6GAL1 in the liver is cleaved and secreted into the serum (8) and may be used as a biomarker for hepatitis diseases (9).

References

Varki, A. et al. (1999) Essentials of Glycobiology, Cold Spring Harbor Laboratory Press, pp195.

Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.

Bast, B.J. et al. (1992) J. Cell Biol. 116:423.
Kitagawa, H. and J.C. Paulson (1994) J. Biol. Chem. 269:17872.
Hennet, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:4504.
Recchi, M.A. et al. (1998) Cancer Res. 58:4066.
Dall’Olio, F. et al. (2001) Eur. J. Biochem. 268:5876.
Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
Kitazume, S. et al. (2009) Glycobiology 19:479.

Long Name

ST6 beta-Galactoside alpha-2,6-Sialyltransferase 1

Entrez Gene IDs

6480 (Human); 20440 (Mouse); 25197 (Rat)

Alternate Names

Alpha 2,6-ST; B-cell antigen CD75; beta-galactoside alpha-2,6-sialyltransferase 1; CD75; CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; EC 2.4.99.1; MGC48859; sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase); sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase); Sialyltransferase 1; SIAT1; SIAT1Alpha 2,6-ST 1; ST6 beta-galactosamide alpha-2,6-sialyltranferase 1; ST6Gal I; ST6GAL1; ST6GalI; ST6N