Home / ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5 / Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody

Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody

Catalog #: MAB67151
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB67151
MAB67151-SP
GalNAc alpha ‑2,6‑sialyltransferase V/ST6GALNAC5 in MDA‑MB‑231 Human Cell Line.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human GalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ST6GALNAC4 or rhST6GALNAC6 is observed.
Source
Monoclonal Mouse IgG2A Clone # 719508
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SGalNAc alpha -2,6-sialyltransferase V/ST6GALNAC5
Gly30-Phe336
Accession # Q9BVH7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 antibody in MDA-MB-231 Human Cell Line by Immunocytochemistry (ICC). View Larger

GalNAc alpha ‑2,6‑sialyltransferase V/ST6GALNAC5 in MDA‑MB‑231 Human Cell Line. GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human GalNAc a-2,6-sialyltransferase V/ST6GALNAC5 Monoclonal Antibody (Catalog # MAB67151) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ST6 GalNAc alpha-2,6-sialyltransferaseV/ST6GALNAC5

Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues (1). They are abundant in the nervous system, where they play crucial modulatory roles in cellular recognition, interaction, adhesion, and signal transduction, particularly during early developmental stages. The expression of gangliosides in the nervous system is developmentally regulated through various sialyltransferases (2). ST6GALNAC5 is a sialyltransferase involved in the biosynthesis of ganglioside GD1a (NeuAc alpha 2,3Gal beta 1,3GalNAc beta 1,4(NeuAc alpha 2,3)Gal beta 1,4Glc beta 1-Cer) from GM1b (NeuAc alpha 2,3Gal beta 1,3GalNAc beta 1,4Gal beta 1,4Glc beta 1-Cer), and its expression is restricted to the brain (3, 4). ST6GALNAC5 has been identified as a key player in the metastasis of breast cancer cells to the brain by potentially enabling the cancer cells to cross the blood-brain barrier (5, 6). The recombinant ST6GALNAC5 was active on fetuin from fetal calf serum when assayed using a phosphatase-coupled method (7) suggesting the substrate specificity of ST6GALNAC5 may require further characterization.

References
  1. Kolter, T. et al. (2002) J. Biol. Chem. 277:25859.
  2. Yu, R.K. et al. (2008) Glycoscience DOI: 10.1007/978-3-540-30429-6_41.
  3. Okajima, T. et al. (1999) J. Biol. Chem.274:30557.
  4. Harduin-Lepers, A. et al. (2005) Glycobiology 15:805.
  5. Bos, P.D. et al. (2009) Nature 459:1005.
  6. Arshad, F. et al. (2011). Patholog. Res. Int. DOI: 10.4061/2011/920509.
  7. Wu, Z.L. et al. (2010) Glycobiology DOI: 10.1093/glycob/cwq187.
Long Name
ST6 alpha-N-acetylGalactosaminide alpha-2,6-sialyltransferase 5
Entrez Gene IDs
81849 (Human); 26938 (Mouse)
Alternate Names
alpha-N-acetylneuraminyl 2,3-betagalactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase E; EC 2.4.99; EC 2.4.99.-; EC 2.4.99.7; GalNAc alpha-2,6-sialyltransferase V; GD1 alpha synthase; MGC3184; sialyltransferase 7 (alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase) E; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase) E; Sialyltransferase 7E; SIAT7E; SIAT7-E; SIAT7Ealpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase V; ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 5; ST6 neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 5; ST6GalNAc V; ST6GALNAC5; ST6GalNAcV; ST6GalNAcValpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The CCL2-CCR2 astrocyte-cancer cell axis in tumor extravasation at the brain
    Authors: Cynthia Hajal, Yoojin Shin, Leanne Li, Jean Carlos Serrano, Tyler Jacks, Roger D. Kamm
    Science Advances

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody

There are currently no reviews for this product. Be the first to review Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody and earn rewards!

Have you used Human ST6 GalNAc alpha-2,6-sialyltransferase V/ST6GALNAC5 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review