Human STAMP2 Antibody

Catalog #: MAB4626 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4626
MAB4626-SP

Detection of STAMP2 in Human STAMP2 Transfected HEK293 Human Cell Line by Flow Cytometry.

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Human STAMP2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human STAMP2-transfected NS0 cells but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 418714

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human STAMP2
Met1-His459
Accession # EAW76907

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of STAMP2 in Human STAMP2 Transfected HEK293 Human Cell Line by Flow Cytometry. Human STAMP2 transfected HEK293 human cell line was stained with Mouse Anti-Human STAMP2 Monoclonal Antibody (Catalog # MAB4626, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAMP2/STEAP4

Human STAMP2, also called Steap4 or TIARP (TNF-alpha -induced adipose-related protein), is a 55 kDa, 459 aa, 6-transmembrane (TM) member of the Steap family of metalloreductases. It is induced by androgens in androgen-regulated prostate cells, and is up‑regulated during adipocyte differentiation by TNF-alpha. Full-length human and mouse STAMP2 share 80% aa identity. A 283 aa variant lacks a portion of the oxidoreductase domain and three TM domains. STAMP2 is present in the Golgi complex and early endosomes in addition to the cell surface.

Long Name

Six Transmembrane Protein of Prostate 2

Entrez Gene IDs

79689 (Human); 117167 (Mouse)

Alternate Names

EC 1.16.1; EC 1.16.1.-; FLJ23153; Six-transmembrane epithelial antigen of prostate 4; SixTransMembrane protein of prostate 2; STAMP2; STAMP2DKFZp666D049; STEAP family member 4; STEAP4; TIARP; TIARPsix transmembrane prostate protein 2; TNFAIP9; Tumor necrosis factor, alpha-induced protein 9metalloreductase STEAP4; tumor necrosis-alpha-induced adipose-related protein