Human STAT6 PE-conjugated Antibody

Name: Human STAT6 PE-conjugated Antibody
Brand: R&D Systems
SKU: IC2167P

Catalog #: IC2167P Datasheet / COA / SDS

Discontinued Product

IC2167P has been discontinued.

View all STAT6 products.

Detection of STAT6 in Jurkat Human Cell Line by Flow Cytometry.

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All STAT6 Antibodies

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Human STAT6 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human STAT6 in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 253906

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human STAT6
Asn341-Gly640
Accession # P42226.1

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of STAT6 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

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Detection of STAT6 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human STAT6 PE-conjugated Monoclonal Antibody (Catalog # IC2167P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT6

STAT family proteins mediate cytokine signaling by acting as signal transducers in the cytoplasm and transcription activators in the nucleus. STAT6 is important for the development of Th2 cells and regulation of gene expression induced by IL-4 and IL-13.

Long Name

Signal Transducer and Activator of Transcription 6

Entrez Gene IDs

6778 (Human); 20852 (Mouse); 362896 (Rat)

Alternate Names

D12S1644; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT