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Human TNF RII/TNFRSF1B ELISA Kit - Quantikine

Catalog #: DRT200
76 Citations 4 Reviews Datasheet / COA / SDS
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SRT200
PDRT200
DRT200
Control Products Available
QC05: Quantikine Immunoassay Control Group 2
Human TNF RII/TNFRSF1B ELISA Cell Culture Supernate/Urine Standard Curve
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Citations (76)
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Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human TNF RII/TNFRSF1B Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours or 4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Citrate Plasma (20 uL), Urine (20 uL)
Sensitivity
2.3 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human sTNF RII
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human TNF RII in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (pg/mL)Range (pg/mL)Standard Deviation (pg/mL)
Serum (n=40)17261003-3170387
EDTA plasma (n=40)1500829-2262320
Heparin plasma (n=40)1678951-2503349
Citrate plasma (n=40)1405787-2145286
Urine* (n=27)1724275-69821274
*The urine values are actual and are not normalized for creatinine content.

Cell Culture Supernates - Human peripheral blood mononuclear cells (1 x 106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate and stimulated with 10 μg/mL of PHA. Aliquots were removed on days 1, 3 and 5 and assayed.

Day 1 (pg/mL)Day 3 (pg/mL)Day 5 (pg/mL)
184128613124




Product Summary

The Quantikine Human sTNF RII Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure human sTNF RII in cell culture supernates, serum, plasma, or urine. It contains E. coli-expressed recombinant human sTNF RII as well as antibodies raised against this polypeptide. The recombinant protein represents the non-glycosylated, N-terminal methionyl form of the naturally occurring human soluble Type II receptor for TNF, minus 53 amino acids from the proline-rich region of the protein just exterior to the transmembrane domain, with an apparent molecular weight of approximately 19.8 kDa. This immunoassay has been shown to accurately quantitate the recombinant soluble sTNF RII. Results obtained using natural sTNF RII showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural sTNF RII. Since the measurement of human sTNF RII by this immunoassay is insensitive to added TNF-alpha or TNF-beta, it is probable that this measurement corresponds to the total amount of the soluble receptor present in samples, (i.e. the total amount of free receptor plus the total amount of receptor bound to TNF).

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 45.3 126 254 97.1 199 430
Standard Deviation 2 3.7 10.4 3.1 6.9 17.8
CV% 4.4 2.9 4.1 3.2 3.5 4.1

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 68.7 179 349 89.8 197 444
Standard Deviation 2.2 4.6 16.8 4.6 6.9 16.2
CV% 3.2 2.6 4.8 5.1 3.5 3.6

Recovery

The recovery of human sTNF RII spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 88-103
Citrate Plasma (n=4) 100 91-116
EDTA Plasma (n=4) 95 85-100
Heparin Plasma (n=4) 89 74-108
Serum (n=4) 97 86-107
Urine (n=4) 97 83-111

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of sTNF RII were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human TNF RII/TNFRSF1B ELISA Linearity

Scientific Data

N/A Human TNF RII/TNFRSF1B ELISA Cell Culture Supernate/Urine Standard Curve View Larger

Human TNF RII/TNFRSF1B ELISA Cell Culture Supernate/Urine Standard Curve

N/A Human TNF RII/TNFRSF1B ELISA Serum/Plasma Standard Curve View Larger

Human TNF RII/TNFRSF1B ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TNF RII/TNFRSF1B

TNF RII (Tumor Necrosis Factor Receptor II), also known as TNFRSF1B, p75/p80, and CD120b, is a widely expressed receptor for membrane-associated TNF-alpha and Lymphotoxin-alpha. Its activation initiates pro-inflammatory and pro-survival responses via NFkB-dependent signaling pathways, although it may also induce apoptosis.

Long Name:
Tumor Necrosis Factor Receptor II
Entrez Gene IDs:
7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)
Alternate Names:
CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 200 µL Standard, Control, or Sample
  4.   Add 200 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process twice for a total of 3 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well.
  7.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    For Cell Culture Supernate Samples & Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
  8.   Aspirate and wash 3 times.
    9. 200 µL Substrate Solution
  9.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human TNF RII/TNFRSF1B Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

76 Citations: Showing 1 - 10
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  1. Impact of TNFRSF1B (rs3397, rs1061624 and rs1061622) and IL6 (rs1800796, rs1800797 and rs1554606) Gene Polymorphisms on Inflammatory Response in Patients with End-Stage Kidney Disease Undergoing Dialysis
    Authors: Coimbra, S;Rocha, S;Catarino, C;Valente, MJ;Rocha-Pereira, P;Sameiro-Faria, M;Oliveira, JG;Madureira, J;Fernandes, JC;Miranda, V;Belo, L;Bronze-da-Rocha, E;Santos-Silva, A;
    Biomedicines
    Species: Human
    Sample Types: Serum
  2. ADAM17 targeting by human cytomegalovirus remodels the cell surface proteome to simultaneously regulate multiple immune pathways
    Authors: Rubina, A;Patel, M;Nightingale, K;Potts, M;Fielding, CA;Kollnberger, S;Lau, B;Ladell, K;Miners, KL;Nichols, J;Nobre, L;Roberts, D;Trinca, TM;Twohig, JP;Vlahava, VM;Davison, AJ;Price, DA;Tomasec, P;Wilkinson, GWG;Weekes, MP;Stanton, RJ;Wang, ECY;
    Proceedings of the National Academy of Sciences of the United States of America
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Hemophagocytic Lymphohistiocytosis With Elevated Cytokines Related to Macrophage Activation After Liver Transplantation for Autoimmune Hepatitis: A Case Report
    Authors: Nakanuma, S;Gabata, R;Okazaki, M;Seki, A;Hosokawa, K;Yokoyama, T;Katano, K;Sugita, H;Tokoro, T;Takada, S;Makino, I;Taniguchi, T;Harada, K;Yagi, S;
    Transplantation proceedings
    Species: Human
    Sample Types: Serum
  4. High molar ratios of tumor necrosis factor ? (TNF ?) soluble receptors I and II to the TNF ligand in human plasma from male veterans with comorbid posttraumatic stress disorder (PTSD) and mild traumatic brain injury (mTBI)
    Authors: Morales, TI;Stamper, CE;Brenner, LA;
    The European journal of psychiatry
    Species: Human
    Sample Types: Plasma
  5. Distinct Plasma Immune Profile in ALS Implicates sTNFR-II in pAMPK/Leptin Homeostasis
    Authors: V Picher-Mar, H Boutej, A Vézina, P Cordeau, H Kaneb, JP Julien, A Genge, N Dupré, J Kriz
    International Journal of Molecular Sciences, 2023-03-07;24(6):.
    Species: Human
    Sample Types: Plasma
  6. Circulating tumor necrosis factor receptors are associated with mortality and disease severity in COVID-19 patients
    Authors: T Gohda, M Murakoshi, Y Suzuki, M Hiki, T Naito, K Takahashi, Y Tabe
    PLoS ONE, 2022-10-11;17(10):e0275745.
    Species: Human
    Sample Types: Serum
  7. Soluble tumor necrosis factor receptor 2 is associated with progressive diabetic kidney disease in patients with type 2 diabetes mellitus
    Authors: TH Wu, LH Chang, CH Chu, CM Hwu, HS Chen, LY Lin
    PLoS ONE, 2022-04-12;17(4):e0266854.
    Species: Human
    Sample Types: Serum
  8. Relationship of Diet to Gut Microbiota and Inflammatory Biomarkers in People with HIV
    Authors: M Manzano, A Talavera-R, E Moreno, N Madrid, MJ Gosalbes, R Ron, F Dronda, JA Pérez-Moli, VF Lanza, J Díaz, S Moreno, B Navia, S Serrano-Vi
    Nutrients, 2022-03-14;14(6):.
    Species: Human
    Sample Types: Plasma
  9. Socioeconomic Status and Inflammation in Women with Early-stage Breast Cancer: Mediation by Body Mass Index
    Authors: YK Pageot, AL Stanton, PA Ganz, MR Irwin, SW Cole, CM Crespi, EC Breen, KR Kuhlman, JE Bower
    Brain, Behavior, and Immunity, 2021-10-18;99(0):307-316.
    Species: Human
    Sample Types: Plasma
  10. Investigating the relationship between particulate matter and inflammatory biomarkers of exhaled breath condensate and blood in healthy young adults
    Authors: M Seifi, N Rastkari, MS Hassanvand, K Naddafi, R Nabizadeh, S Nazmara, H Kashani, A Zare, Z Pourpak, SY Hashemi, M Yunesian
    Scientific Reports, 2021-06-21;11(1):12922.
    Species: Human
    Sample Types: Serum
  11. IL-6, sTNF-R2, and CRP in the context of sleep, circadian preference, and health in adolescents with eveningness chronotype: Cross-sectional and longitudinal treatment effects
    Authors: MR Dolsen, AG Harvey
    Psychoneuroendocrinology, 2021-04-26;129(0):105241.
    Species: Human
    Sample Types: Oral mucosal transudate
  12. Combination of subtherapeutic anti-TNF dose with dasatinib restores clinical and molecular arthritogenic profiles better than standard anti-TNF treatment
    Authors: L Ntari, C Nikolaou, K Kranidioti, D Papadopoul, E Christodou, P Chouvardas, F Meier, C Geka, MC Denis, N Karagianni, G Kollias
    Journal of Translational Medicine, 2021-04-23;19(1):165.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Fecal microbiota transplantation in HIV: A pilot placebo-controlled study
    Authors: S Serrano-Vi, A Talavera-R, MJ Gosalbes, N Madrid, JA Pérez-Moli, RJ Elliott, B Navia, VF Lanza, A Vallejo, M Osman, F Dronda, S Budree, J Zamora, C Gutiérrez, M Manzano, MJ Vivancos, R Ron, J Martínez-S, S Herrera, U Ansa, A Moya, S Moreno
    Nature Communications, 2021-02-18;12(1):1139.
    Species: Human
    Sample Types: Plasma
  14. Comparison of Circulating Biomarkers in Predicting Diabetic Kidney Disease Progression With Autoantibodies to Erythropoietin Receptor
    Authors: M Oshima, A Hara, T Toyama, M Jun, C Pollock, M Jardine, S Harrap, N Poulter, ME Cooper, M Woodward, J Chalmers, V Perkovic, MG Wong, T Wada
    Kidney international reports, 2020-11-10;6(2):284-295.
    Species: Human
    Sample Types: Plasma
  15. Circulating Tumor Necrosis Factor Receptors: A Potential Biomarker for the Progression of Diabetic Kidney Disease
    Authors: M Murakoshi, T Gohda, Y Suzuki
    Int J Mol Sci, 2020-03-13;21(6):.
    Species: Human
    Sample Types: Plasma
  16. Determinants of low bone mineral density in people with multiple sclerosis: Role of physical activity
    Authors: BT Cleland, P Papanek, BA Ingraham, A Harkins, M Garnier-Vi, D Woo, ME Csuka, A V Ng
    Mult Scler Relat Disord, 2019-11-23;38(0):101864.
    Species: Human
    Sample Types: Serum
  17. Correlation between biomarkers of pain in saliva and PAINAD scale in elderly people with cognitive impairment and inability to communicate: descriptive study protocol
    Authors: V Cantón-Hab, MDP Carrera-Go, MT Moreno-Cas, JM Quesada-Gó, M Rich-Ruiz
    BMJ Open, 2019-11-10;9(11):e032927.
    Species: Human
    Sample Types: Saliva
  18. Systemic Inflammation Precedes Microalbuminuria in Diabetes
    Authors: FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk
    Kidney Int Rep, 2019-06-21;4(10):1373-1386.
    Species: Human
    Sample Types: Serum
  19. Interference of tumor necrosis factor inhibitor treatments on soluble tumor necrosis factor receptor 2 levels in rheumatoid arthritis
    Authors: N Yang, J Huang, M Frits, C Iannaccone, ME Weinblatt, N Rifai, N Shadick, G Bradwin, KP Liao
    Pract Lab Med, 2019-05-03;16(0):e00122.
    Species: Human
    Sample Types: Plasma
  20. Tumor necrosis factor-alpha and Fas/Fas ligand signaling pathways in chronic spontaneous urticaria
    Authors: R Grzanka, A Damasiewic, A Kasperska-
    Allergy Asthma Clin Immunol, 2019-03-14;15(0):15.
    Species: Human
    Sample Types: Serum
  21. Elevated Inflammatory Plasma Biomarkers in Patients With Fabry Disease: A Critical Link to Heart Failure With Preserved Ejection Fraction
    Authors: H Yogasundar, A Nikhanj, BN Putko, M Boutin, S Jain-Ghai, A Khan, C Auray-Blai, ML West, GY Oudit
    J Am Heart Assoc, 2018-11-06;7(21):e009098.
    Species: Human
    Sample Types: Plasma
  22. Plasma Levels of Soluble Tumor Necrosis Factor Receptor 2 (sTNFR2) are Associated with Hippocampal Volume and Cognitive Performance in Patients with Schizophrenia
    Authors: N Kudo, H Yamamori, T Ishima, K Nemoto, Y Yasuda, M Fujimoto, H Azechi, T Niitsu, S Numata, M Ikeda, M Iyo, T Ohmori, M Fukunaga, Y Watanabe, K Hashimoto, R Hashimoto
    Int. J. Neuropsychopharmacol., 2018-07-01;0(0):.
    Species: Human
    Sample Types: Plasma
  23. Plasma inflammatory cytokines and survival of pancreatic cancer patients
    Authors: A Babic, N Schnure, NP Neupane, MM Zaman, N Rifai, MW Welch, LK Brais, DA Rubinson, V Morales-Oy, C Yuan, S Zhang, EM Poole, BM Wolpin, MH Kulke, DA Barbie, K Wong, CS Fuchs, K Ng
    Clin Transl Gastroenterol, 2018-04-25;9(4):145.
    Species: Human
    Sample Types: Plasma
  24. Reevaluation of immune activation in the era of cART and an aging HIV-infected population
    Authors: LR de Armas, S Pallikkuth, V George, S Rinaldi, R Pahwa, KL Arheart, S Pahwa
    JCI Insight, 2017-10-19;2(20):.
    Species: Human
    Sample Types: Plasma
  25. Bioaerosol exposure and circulating biomarkers in a panel of elderly subjects and healthy young adults
    Authors: S Faridi, K Naddafi, H Kashani, R Nabizadeh, M Alimohamma, F Momeniha, S Faridi, S Niazi, A Zare, A Gholampour, M Hoseini, Z Pourpak, MS Hassanvand, M Yunesian
    Sci. Total Environ, 2017-03-27;593(0):380-389.
    Species: Human
    Sample Types: Plasma
  26. Circulating TNF Receptors 1 and 2 Predict Mortality in Patients with End-stage Renal Disease Undergoing Dialysis
    Authors: T Gohda, S Maruyama, N Kamei, S Yamaguchi, T Shibata, M Murakoshi, S Horikoshi, Y Tomino, I Ohsawa, H Gotoh, S Nojiri, Y Suzuki
    Sci Rep, 2017-03-03;7(0):43520.
    Species: Human
    Sample Types: Serum
  27. Soluble Tumor Necrosis Factor Receptors and Arterial Stiffness in Patients With Coronary Atherosclerosis
    Authors: Hack-Lyoung Kim
    Am. J. Hypertens, 2017-03-01;0(0):.
    Species: Human
    Sample Types: Plasma
  28. Short-term effects of particle size fractions on circulating biomarkers of inflammation in a panel of elderly subjects and healthy young adults
    Authors: MS Hassanvand, K Naddafi, H Kashani, S Faridi, N Kunzli, R Nabizadeh, F Momeniha, A Gholampour, M Arhami, A Zare, Z Pourpak, M Hoseini, M Yunesian
    Environ. Pollut, 2017-02-10;223(0):695-704.
    Species: Human
    Sample Types: Plasma
  29. Effects of an Encapsulated Fruit and Vegetable Juice Concentrate on Obesity-Induced Systemic Inflammation: A Randomised Controlled Trial
    Authors: EJ Williams, KJ Baines, BS Berthon, LG Wood
    Nutrients, 2017-02-08;9(2):.
    Species: Human
    Sample Types: Plasma
  30. Anti-TNF-? Drugs Differently Affect the TNF?-sTNFR System and Monocyte Subsets in Patients with Psoriasis
    PLoS ONE, 2016-12-09;11(12):e0167757.
    Species: Human
    Sample Types: Plasma
  31. Prevalent peripheral arterial disease and inflammatory burden
    BMC Geriatr, 2016-12-09;16(1):213.
    Species: Human
    Sample Types: Serum
  32. Reliability of Potential Pain Biomarkers in the Saliva of Healthy Subjects: Inter-Individual Differences and Intersession Variability
    PLoS ONE, 2016-12-01;11(12):e0166976.
    Species: Human
    Sample Types: Saliva
  33. Altered Monocyte and Endothelial Cell Adhesion Molecule Expression Is Linked to Vascular Inflammation in Human Immunodeficiency Virus Infection
    Authors: M Kulkarni, E Bowman, J Gabriel, T Amburgy, E Mayne, DA Zidar, C Maierhofer, AN Turner, JA Bazan, SL Koletar, MM Lederman, SF Sieg, NT Funderburg
    Open Forum Infect Dis, 2016-10-15;3(4):ofw224.
    Species: Human
    Sample Types: Plasma
  34. Anti-Drug Antibodies, Drug Levels, Interleukin-6 and Soluble TNF Receptors in Rheumatoid Arthritis Patients during the First 6 Months of Treatment with Adalimumab or Infliximab: A Descriptive Cohort Study
    PLoS ONE, 2016-09-08;11(9):e0162316.
    Species: Human
    Sample Types: Serum
  35. Local TNF causes NFATc1-dependent cholesterol-mediated podocyte injury
    J Clin Invest, 2016-08-02;0(0):.
    Species: Human
    Sample Types: Serum
  36. Inflammatory Markers and the Risk of Hip and Vertebral Fractures in Men: the Osteoporotic Fractures in Men (MrOS)
    J Bone Miner Res, 2016-07-25;0(0):.
    Species: Human
    Sample Types: Serum
  37. Stability of cytokines, chemokines and soluble activation markers in unprocessed blood stored under different conditions
    Cytokine, 2016-05-18;84(0):17-24.
    Species: Human
    Sample Types: Plasma
  38. Association between serum soluble tumor necrosis factor-? receptors and early childhood obesity
    Authors: S Ouyang, W Li, Z Liu, Y Li, S Li, J Wu
    Endocr J, 2016-03-31;0(0):.
    Species: Human
    Sample Types: Serum
  39. Late Antiretroviral Therapy (ART) Initiation Is Associated with Long-Term Persistence of Systemic Inflammation and Metabolic Abnormalities.
    Authors: Ghislain M, Bastard J, Meyer L, Capeau J, Fellahi S, Gerard L, May T, Simon A, Vigouroux C, Goujard C
    PLoS ONE, 2015-12-04;10(12):e0144317.
    Species: Human
    Sample Types: Serum
  40. Plasma TNF-alpha and Soluble TNF Receptor Levels after Doxorubicin with or without Co-Administration of Mesna-A Randomized, Cross-Over Clinical Study.
    Authors: Hayslip J, Dressler E, Weiss H, Taylor T, Chambers M, Noel T, Miriyala S, Keeney J, Ren X, Sultana R, Vore M, Butterfield D, St Clair D, Moscow J
    PLoS ONE, 2015-04-24;10(4):e0124988.
    Species: Human
    Sample Types: Plasma
  41. Circulating TNF receptors 1 and 2 are associated with the severity of renal interstitial fibrosis in IgA nephropathy.
    Authors: Sonoda Y, Gohda T, Suzuki Y, Omote K, Ishizaka M, Matsuoka J, Tomino Y
    PLoS ONE, 2015-04-10;10(4):e0122212.
    Species: Human
    Sample Types: Serum
  42. A case-control study of pre-operative levels of serum neutrophil gelatinase-associated lipocalin and other potential inflammatory markers in colorectal cancer.
    Authors: Duvillard L, Ortega-Deballon P, Bourredjem A, Scherrer M, Mantion G, Delhorme J, Deguelte-Lardiere S, Petit J, Bonithon-Kopp C
    BMC Cancer, 2014-12-03;14(0):912.
    Species: Human
    Sample Types: Serum
  43. Circulating levels of tumor necrosis factor-alpha receptor 2 are increased in heart failure with preserved ejection fraction relative to heart failure with reduced ejection fraction: evidence for a divergence in pathophysiology.
    Authors: Putko B, Wang Z, Lo J, Anderson T, Becher H, Dyck J, Kassiri Z, Oudit G
    PLoS ONE, 2014-06-12;9(6):e99495.
    Species: Human
    Sample Types: Plasma
  44. Synergism between circulating tumor necrosis factor receptor 2 and HbA(1c) in determining renal decline during 5-18 years of follow-up in patients with type 1 diabetes and proteinuria.
    Authors: Skupien J, Warram J, Niewczas M, Gohda T, Malecki M, Mychaleckyj J, Galecki A, Krolewski A
    Diabetes Care, 2014-06-04;37(9):2601-8.
    Species: Human
    Sample Types: Serum
  45. Effect of IL-6 on the insulin sensitivity in patients with type 2 diabetes.
    Authors: Harder-Lauridsen N, Krogh-Madsen R, Holst J, Plomgaard P, Leick L, Pedersen B, Fischer C
    Am J Physiol Endocrinol Metab, 2014-01-28;306(7):E769-78.
    Species: Human
    Sample Types: Plasma
  46. Compensated inflammation in systemic juvenile idiopathic arthritis: role of alternatively activated macrophages.
    Authors: Shimizu M, Yachie A
    Cytokine, 2012-06-08;60(1):226-32.
    Species: Human
    Sample Types: Serum
  47. Inflammatory markers and the risk of hip fracture: the Women's Health Initiative.
    Authors: Barbour KE, Boudreau R, Danielson ME, Youk AO, Wactawski-Wende J, Greep NC, LaCroix AZ, Jackson RD, Wallace RB, Bauer DC, Allison MA, Cauley JA
    J. Bone Miner. Res., 2012-05-01;27(5):1167-76.
    Species: Human
    Sample Types: Serum
  48. Inflammation marker and risk of pancreatic cancer: a nested case-control study within the EPIC cohort.
    Authors: Grote VA, Kaaks R, Nieters A, Tjonneland A, Halkjaer J, Overvad K, Skjelbo Nielsen MR, Boutron-Ruault MC, Clavel-Chapelon F, Racine A, Teucher B, Becker S, Pischon T, Boeing H, Trichopoulou A, Cassapa C, Stratigakou V, Palli D, Krogh V, Tumino R, Vineis P, Panico S, Rodriguez L, Duell EJ, Sanchez MJ, Dorronsoro M, Navarro C, Gurrea AB, Siersema PD, Peeters PH, Ye W, Sund M, Lindkvist B, Johansen D, Khaw KT, Wareham N, Allen NE, Travis RC, Fedirko V, Jenab M, Michaud DS, Chuang SC, Romaguera D, Bueno-de-Mesquita HB, Rohrmann S
    Br. J. Cancer, 2012-04-26;106(11):1866-74.
    Species: Human
    Sample Types: Serum
  49. Negative and competitive social interactions are related to heightened proinflammatory cytokine activity.
    Authors: Chiang JJ, Eisenberger NI, Seeman TE, Taylor SE
    Proc. Natl. Acad. Sci. U.S.A., 2012-01-23;109(6):1878-82.
    Species: Human
    Sample Types: Plasma
  50. Elevated CSF levels of TACE activity and soluble TNF receptors in subjects with mild cognitive impairment and patients with Alzheimer's disease.
    Authors: Jiang H, Hampel H, Prvulovic D, Wallin A, Blennow K, Li R, Shen Y
    Mol Neurodegener, 2011-10-06;6(0):69.
    Species: Human
    Sample Types: CSF
  51. Procalcitonin as an early marker of bacterial infection in neutropenic febrile children with acute lymphoblastic leukemia.
    Authors: Hatzistilianou M, Rekliti A, Athanassiadou F, Catriu D
    Inflamm. Res., 2009-10-06;59(5):339-47.
    Species: Human
    Sample Types: Serum
  52. Adrenomedullin and endothelin-1 are related to inflammation in chronic heart failure.
    Authors: Gombos T, Forhecz Z, Pozsonyi Z, Wallentin S, Papassotiriou J, Kunde J, Morgenthaler NG, Janoskuti L, Prohaszka Z
    Inflamm. Res., 2009-06-01;58(6):298-305.
    Species: Human
    Sample Types: Serum
  53. Biomarkers of inflammation and development of rheumatoid arthritis in women from two prospective cohort studies.
    Authors: Karlson EW, Chibnik LB, Tworoger SS, Lee IM, Buring JE, Shadick NA, Manson JE, Costenbader KH
    Arthritis Rheum., 2009-03-01;60(3):641-52.
    Species: Human
    Sample Types: Plasma
  54. Cross-sectional relations of multiple inflammatory biomarkers to peripheral arterial disease: The Framingham Offspring Study.
    Authors: Murabito JM, Keyes MJ, Guo CY, Keaney JF, Vasan RS, D'Agostino RB, Benjamin EJ
    Atherosclerosis, 2008-07-09;203(2):509-14.
    Species: Human
    Sample Types: Plasma
  55. Neurobiological correlates of coping through emotional approach.
    Authors: Master SL, Amodio DM, Stanton AL, Yee CM, Hilmert CJ, Taylor SE
    Brain Behav. Immun., 2008-06-16;23(1):27-35.
    Species: Human
    Sample Types: Saliva
  56. Comparing Type D personality and older age as correlates of tumor necrosis factor-alpha dysregulation in chronic heart failure.
    Authors: Denollet J, Vrints CJ, Conraads VM
    Brain Behav. Immun., 2008-02-20;22(5):736-43.
    Species: Human
    Sample Types: Plasma
  57. Inflammatory markers and incident fracture risk in older men and women: the Health Aging and Body Composition Study.
    Authors: Cauley JA, Danielson ME, Boudreau RM, Forrest KY, Zmuda JM, Pahor M, Tylavsky FA, Cummings SR, Harris TB, Newman AB
    J. Bone Miner. Res., 2007-07-01;22(7):1088-95.
    Species: Human
    Sample Types: Serum
  58. Selective functional exhaustion of hematopoietic progenitor cells in the bone marrow of patients with postinfarction heart failure.
    Authors: Kissel CK, Lehmann R, Assmus B, Aicher A, Honold J, Fischer-Rasokat U, Heeschen C, Spyridopoulos I, Dimmeler S, Zeiher AM
    J. Am. Coll. Cardiol., 2007-06-04;49(24):2341-9.
    Species: Human
    Sample Types: Serum
  59. Muscle atrophy and hypertrophy signaling in patients with chronic obstructive pulmonary disease.
    Authors: Doucet M, Russell AP, Leger B, Debigare R, Joanisse DR, Caron MA, LeBlanc P, Maltais F
    Am. J. Respir. Crit. Care Med., 2007-05-03;176(3):261-9.
    Species: Human
    Sample Types: Plasma
  60. Serum interleukin-18 and soluble tumour necrosis factor receptor 2 are associated with disease severity in patients with paracoccidioidomycosis.
    Authors: Corvino CL, Mamoni RL, Fagundes GZ, Blotta MH
    Clin. Exp. Immunol., 2007-03-01;147(3):483-90.
    Species: Human
    Sample Types: Serum
  61. The shedding activity of ADAM17 is sequestered in lipid rafts.
    Authors: Tellier E, Rebsomen L
    Exp. Cell Res., 2006-09-05;312(20):3969-80.
    Species: Primate - Cholrocebus pygerythrus (Vervet Monkey)
    Sample Types: Cell Lysates
  62. Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
    Authors: Ebert EC, Mehta V
    Cell. Immunol., 2006-08-17;241(1):7-13.
    Species: Human
    Sample Types: Cell Culture Supernates
  63. Proinflammatory cytokines, insulin resistance, and insulin secretion in chronic hepatitis C patients: A case-control study.
    Authors: Lecube A, Hernandez C, Genesca J, Simo R
    Diabetes Care, 2006-05-01;29(5):1096-101.
    Species: Human
    Sample Types: Serum
  64. Experimental heatstroke in baboon: analysis of the systemic inflammatory response.
    Authors: Bouchama A, Ollivier V, Roberts G, Al Mohanna F, de Prost D, Eldali A, Saussereau E, El-Sayed R, Chollet-Martin S
    Shock, 2005-10-01;24(4):332-5.
    Species: Primate - Papio hamadryas (Hamadyras Baboon)
    Sample Types: Plasma
  65. Regulation of peritoneal and systemic neutrophil-derived tumor necrosis factor-alpha release in patients with severe peritonitis: role of tumor necrosis factor-alpha converting enzyme cleavage.
    Authors: Kermarrec N, Selloum S, Plantefeve G, Chosidow D, Paoletti X, Lopez A, Mantz J, Desmonts JM, Gougerot-Pocidalo MA, Chollet-Martin S
    Crit. Care Med., 2005-06-01;33(6):1359-64.
    Species: Human
    Sample Types: Cell Culture Supernates
  66. Growth hormone-induced reduction of soluble apoptosis mediators is associated with reverse cardiac remodelling and improvement of exercise capacity in patients with idiopathic dilated cardiomyopathy.
    Authors: Parissis JT, Adamopoulos S, Karatzas D, Paraskevaidis J, Livanis E, Kremastinos D
    Eur J Cardiovasc Prev Rehabil, 2005-04-01;12(2):164-8.
    Species: Human
    Sample Types: Plasma
  67. Levels of soluble cytokine factors in temporomandibular joint effusions seen on magnetic resonance images.
    Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005-04-01;99(4):411-8.
    Species: Human
    Sample Types: Synovial Fluid
  68. Analysis of tumor necrosis factor-alpha, interleukin-6, interleukin-1beta, soluble tumor necrosis factor receptors I and II, interleukin-6 soluble receptor, interleukin-1 soluble receptor type II, interleukin-1 receptor antagonist, and protein in the synovial fluid of patients with temporomandibular joint disorders.
    Authors: Kaneyama K, Segami N, Sun W, Sato J, Fujimura K
    Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2005-03-01;99(3):276-84.
    Species: Human
    Sample Types: Synovial Fluid
  69. Changes in HIV RNA viral load, CD4+ T-cell counts, and levels of immune activation markers associated with anti-tuberculosis therapy and cotrimoxazole prophylaxis among HIV-infected tuberculosis patients in Abidjan, Cote d'Ivoire.
    Authors: Kalou M, Sassan-Morokro M, Abouya L, Bile C, Maurice C, Maran M, Tossou O, Roels T, Greenberg AE, Wiktor SZ, Nkengasong JN
    J. Med. Virol., 2005-02-01;75(2):202-8.
    Species: Human
    Sample Types: Serum
  70. Immunological effects of induced shame and guilt.
    Authors: Dickerson SS, Kemeny ME, Aziz N, Kim KH, Fahey JL
    Psychosom Med, 2004-01-01;66(1):124-31.
    Species: Human
    Sample Types: Saliva
  71. Pro- and anti-inflammatory responses in respiratory syncytial virus bronchiolitis.
    Authors: McNamara PS, Flanagan BF, Selby AM, Hart CA, Smyth RL
    Eur. Respir. J., 2004-01-01;23(1):106-12.
    Species: Human
    Sample Types: BALF
  72. Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
    Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
    J. Virol., 2003-02-01;77(3):1727-37.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Serum
  73. Functional and phenotypic changes in circulating lymphocytes from hospitalized zambian children with measles.
    Authors: Ryon JJ, Moss WJ, Monze M, Griffin DE
    Clin. Diagn. Lab. Immunol., 2002-09-01;9(5):994-1003.
    Species: Human
    Sample Types: Plasma
  74. Age-related autoantibody production in a nonhuman primate model.
    Authors: Attanasio R, Brasky KM, Robbins SH, Jayashankar L, Nash RJ, Butler TM
    Clin. Exp. Immunol., 2001-03-01;123(3):361-5.
    Species: Primate - Papio cynocephalus (Yellow Baboon)
    Sample Types: Serum
  75. An analysis of 14 molecular markers for monitoring osteoarthritis: segregation of the markers into clusters and distinguishing osteoarthritis at baseline.
    Authors: Otterness IG, Swindell AC, Zimmerer RO, Poole AR, Ionescu M, Weiner E
    Osteoarthr. Cartil., 2000-05-01;8(3):180-5.
    Species: Human
    Sample Types: Serum
  76. Tumour necrosis factor alpha and its soluble receptors in juvenile chronic arthritis.
    Authors: Rooney M, Varsani H, Martin K, Lombard PR, Dayer JM, Woo P
    Rheumatology (Oxford), 2000-04-01;39(4):432-8.
    Species: Human
    Sample Types: Serum

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Human TNF RII/TNFRSF1B Quantikine ELISA Kit
By Anonymous on 11/10/2023
Sample Tested: Urine

Urine showed good linearity up to 160-fold dilution.

Human TNF RII/TNFRSF1B Quantikine ELISA Kit DRT200
Human TNF RII/TNFRSF1B Quantikine ELISA Kit
By James Williams on 10/13/2021
Sample Tested: EDTA Plasma
Human TNF RII/TNFRSF1B Quantikine ELISA Kit DRT200
Human sTNF RII/TNFRSF1B Quantikine ELISA Kit
By Anonymous on 02/03/2021
Sample Tested: Plasma

Kit performed as intended with very clear protocol.

Human sTNF RII/TNFRSF1B Quantikine ELISA Kit DRT200
Human sTNF RII/TNFRSF1B Quantikine ELISA Kit
By Anonymous on 12/06/2019
Sample Tested: Jurkat human acute T cell leukemia cell line