Human STYK1 Antibody

Catalog #: MAB6668 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6668
MAB6668-SP

Detection of Human STYK1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human STYK1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human STYK1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 484713

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human STYK1
Asp72-Val320
Accession # Q6J9G0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human STYK1 by Western Blot. Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, DLD clone 2C2 human colon adenocarcinoma cell line, and T47D human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human STYK1 Monoclonal Antibody (Catalog # MAB6668) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STYK1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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STYK1 in PC‑3 Human Cell Line. STYK1 was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human STYK1 Monoclonal Antibody (Catalog # MAB6668) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry

Detection of STYK1 antibody in HeLa Human Cell Line antibody by Flow Cytometry.

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Detection of STYK1 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human STYK1 Monoclonal Antibody (Catalog # MAB6668, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STYK1

STYK1 (Ser/Thr/Tyr kinase 1; also NOK, or “novel oncogene with kinase-domain”) is a 50 kDa, distant member of the FGFR/PDGFR family of tyrosine kinases. It appears to be intracellular, even though it contains a putative transmembrane segment. Both MAPK and PI3K pathways are activated by STYK1 activity, and STYK1 is associated with oncogenesis. Human STYK1 is 422 amino acids (aa) in length. It contains 25 aa N-terminus, a 21 aa putative transmembrane segment, and a 396 aa C-terminus that contains a Tyr-kinase domain (aa 118-372). Over aa 72-320, human STYK1 is 77% and 83% identical to mouse and canine STYK1, respectively.

Long Name

Serine/Threonine/Tyrosine Kinase 1

Entrez Gene IDs

55359 (Human); 243659 (Mouse); 500340 (Rat)

Alternate Names

DKFZp761P1010; EC 2.7.10.2; NOK; NOKProtein PK-unique; serine/threonine/tyrosine kinase 1Novel oncogene with kinase domain; STYK1; SuRTK106; tyrosine-protein kinase STYK1