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Human Syndecan-2/CD362 APC-conjugated Antibody

Catalog #: FAB2965A
12 Citations Datasheet / COA / SDS
Clone 305515 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB2965A
Detection of Syndecan-2/CD362 in Human Blood Monocytes by Flow Cytometry.
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Product Details
Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Syndecan-2/CD362 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Syndecan-2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Syndecan-1, recombinant human (rh) Syndecan-3, or rhSyndecan-4 is observed.
Source
Monoclonal Rat IgG2B Clone # 305515
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Syndecan-2
Glu19-Gly144
Accession # AAH49836.1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Syndecan-2/CD362 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of Syndecan-2/CD362 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Rat Anti-Human Syndecan-2/CD362 APC-conjugated Monoclonal Antibody (Catalog # FAB2965A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Syndecan-2/CD362 antibody in Colo205 cells antibody by Flow Cytometry. View Larger

Detection of Syndecan-2/CD362 in Colo205 cells by Flow Cytometry. COLO 205 human colorectal adenocarcinoma cell line was stained with Rat Anti-Human Syndecan-2/CD362 APC-conjugated Monoclonal Antibody (Catalog # FAB2965A, filled histogram) or Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Syndecan-2/CD362

Syndecan-2, previously known as fibroglycan or heparan sulfate proteoglycan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans. The four vertebrate syndecans show conserved cytoplasmic domains and divergent extracellular portions (except for GAG attachment sites). Among the Syndecans, Syndecan-2 is most similar to Syndecan-4 (1‑3). Human Syndecan-2 is synthesized as a 201 amino acid (aa) core protein with an 18 aa signal sequence, a 126 aa extracellular domain (ECD), a 25 aa transmembrane region and a 32 aa cytoplasmic tail (4). The human ECD of Syndecan-2 contains three closely-spaced consensus Ser-Gly sequences for the attachment of HS side chains. It shares 76%, 73%, 87%, 78% and 63% aa identity with the ECD of mouse, rat, bovine, canine and chicken Syndecan-2, respectively. The cytoplasmic tail has both serine and tyrosine phosphorylation sites. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 22 kDa core protein. Non-covalent homodimerization of Syndecan-2 is dependent on the transmembrane domain (5). Syndecan-2 is expressed in cells of mesenchymal origin, neuronal and epithelial cells, and is the predominant syndecan expressed during embryonic development. Expression is upregulated in several cancer cell lines (6). After induction in macrophages by inflammatory mediators, Syndecan-2 selectively binds FGF basic, VEGF and EGF (7). Syndecan-2 expressed on human primary osteoblasts binds GM-CSF and may function as a co‑receptor (8). Activated endothelial cell Syndecan-2 specifically binds IL-8 and may participate in promoting neutrophil extravasation by forming a chemotactic IL-8 gradient (9). Typically, cytokine, chemokine and extracellular matrix protein binding occurs through interaction with HS side chains, but the Syndecan-2 extracellular domain can bind TGF-beta directly via protein-protein interaction (10).

References
  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
  3. Essner, J. J. et al. (2006) Int. J. Biochem. Cell Biol. 38:152.
  4. Marynen, P. et al. (1989) J. Biol. Chem. 264:7017.
  5. Choi, S. et al. (2005) J. Biol. Chem. 280:42573.
  6. Park, H. et al. (2002) J. Biol. Chem. 277:29730.
  7. Clasper, S. et al. (1999) J. Biol. Chem. 274:24113.
  8. Modrowski, D. et al. (2000) J. Biol. Chem. 275:9178.
  9. Halden, Y. et al. (2004) Biochem. J. 377:533.
  10. Chen, L. et al. (2004) J. Biol. Chem. 279:15715.
Entrez Gene IDs
6383 (Human); 15529 (Mouse)
Alternate Names
CD362 antigen; CD362; Fibroglycan; heparan sulfate proteoglycan 1, cell surface-associated; Heparan sulfate proteoglycan core protein; HSPG; HSPG1; HSPG1syndecan proteoglycan 2; SDC2; SYND2; SYND2cell surface-associated heparan sulfate proteoglycan 1; syndecan 2; Syndecan2; Syndecan-2

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Citations for Human Syndecan-2/CD362 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. SDC2 Stabilization by USP14 Promotes Gastric Cancer Progression through Co-option of PDK1
    Authors: You, L;Dou, Y;Zhang, Y;Xiao, H;Lv, H;Wei, GH;Xu, D;
    International journal of biological sciences
  2. SDC2 Stabilization by USP14 Promotes Gastric Cancer Progression through Co-option of PDK1
    Authors: You, L;Dou, Y;Zhang, Y;Xiao, H;Lv, H;Wei, GH;Xu, D;
    International journal of biological sciences
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Flow Cytometry
  3. Contribution of Syndecans to the Cellular Entry of SARS-CoV-2
    Authors: A Hudák, A Letoha, L Szilák, T Letoha
    International Journal of Molecular Sciences, 2021-05-19;22(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Contribution of syndecans to cellular internalization and fibrillation of amyloid-?(1-42)
    Authors: T Letoha, A Hudák, E Kusz, A Pettkó-Sza, I Domonkos, K Jósvay, M Hofmann-Ap, L Szilák
    Sci Rep, 2019-02-04;9(1):1393.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. PAX7 Targets, CD54, Integrin alpha9beta1, and SDC2, Allow Isolation of Human ESC/iPSC-Derived Myogenic Progenitors.
    Authors: Magli A, Incitti T, Kiley J, Swanson S, Darabi R, Rinaldi F, Selvaraj S, Yamamoto A, Tolar J, Yuan C, Stewart R, Thomson J, Perlingeiro R
    Cell Rep, 2017-06-27;19(13):2867-2877.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease
    Authors: SFH de Witte, AM Merino, M Franquesa, T Strini, JAA van Zoggel, SS Korevaar, F Luk, M Gargesha, L O'Flynn, D Roy, SJ Elliman, PN Newsome, CC Baan, MJ Hoogduijn
    Stem Cell Res Ther, 2017-06-08;8(1):140.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Aging of bone marrow- and umbilical cord-derived mesenchymal stromal cells during expansion.
    Authors: de Witte S, Lambert E, Merino A, Strini T, Douben H, O'Flynn L, Elliman S, de Klein A, Newsome P, Baan C, Hoogduijn M
    Cytotherapy, 2017-04-24;19(7):798-807.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Molecular mechanisms linking high dose medroxyprogesterone with HIV-1 risk.
    Authors: Irvin S, Herold B
    PLoS ONE, 2015-03-23;10(3):e0121135.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Hemodynamic shear stress characteristic of atherosclerosis-resistant regions promotes glycocalyx formation in cultured endothelial cells.
    Authors: Koo A, Dewey C, Garcia-Cardena G
    Am J Physiol Cell Physiol, 2012-10-31;304(2):C137-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Targeted inhibition of T-cell factor activity promotes syndecan-2 expression and sensitization to doxorubicin in osteosarcoma cells and bone tumors in mice.
    J. Bone Miner. Res., 2012-10-01;27(10):2118-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1.
    BMC Cancer, 2012-05-14;12(0):176.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry.
    Authors: Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P
    Immunol. Lett., 2010-10-15;134(2):113-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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