Human Syndecan-3 Isoform 1 Antibody

Catalog #: AF3539
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3539
AF3539-SP

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Citations (4)

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Human Syndecan-3 Isoform 1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Syndecan-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse Syndecan-3 is observed and less than 1% cross-reactivity with recombinant mouse Syndecan-4 and recombinant human Syndecan-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Syndecan-3
Gln48-Lys383
Accession # O75056

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Syndecan‑3 (Catalog # 3539-SD)

Flow Cytometry

2.5 µg/10⁶ cells

A172 human glioblastoma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

Immersion fixed IMR‑90 human lung fibroblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Syndecan‑3 in IMR‑90 Human Cell Line. Syndecan‑3 was detected in immersion fixed IMR‑90 human lung fibroblast cell line (positive staining) and HL‑60 human acute promyelocytic leukemia cell line (negative staining) using Goat Anti-Human Syndecan‑3 Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3539) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-3

Syndecan-3, also called N-syndecan, is one of four vertebrate syndecans that are principal carriers of heparan sulfate and chondroitin sulfate glycosaminoglycans (GAGs) (1‑3). These type 1 transmembrane proteins show conserved cytoplasmic domains and divergent extracellular domains (1‑3). Human Syndecan-3 is synthesized as a 442 amino acid (aa) core protein with a 44 aa signal sequence, a 343 aa extracellular domain (ECD), a 21 aa transmembrane (TM) region and a 34 aa cytoplasmic tail with a binding site for PDZ domains (1). The ECD of human Syndecan-3 shares 83%, 83%, 92%, 91% and 91% aa identity with of mouse, rat, equine, bovine and canine Syndecan-3, respectively. Splice isoforms of 384 aa and 346 aa, containing either a 28 aa substitution for aa 1‑86 or deletion of aa 1‑96, have been reported (4). Syndecan-3 contains four conserved closely-spaced GAG attachment sites near the N-terminus and unique threonine-rich and mucin-like sequences near the membrane (4). Addition of glycan side chains results in an apparent size of 120‑150 kDa. Non-covalent homodimerization of Syndecan-3 or, potentially, heterodimerization with Syndecan-2 or -4, is dependent on the transmembrane domain (5). A cleavage site near the TM domain allows shedding of soluble ECD; the remainder of the molecule undergoes regulated intramembrane proteolysis (6). Syndecan-3 is expressed in the nervous system and at limb buds during development (1, 2). It is expressed on neuronal axons and Schwann cell perinodal processes, promoting nerve cell migration and synapse formation (7, 8). Roles in memory and body weight regulation have been described (2, 9, 10). Through localization of growth factors such as FGF2, HGF and TGF-beta, it regulates expression of molecules important for differentiation of muscle and bone, such as myogenin, BMP-2 and hedgehog family members (1, 2, 11‑13). In adults, it is upregulated during regeneration, such as following myocardial infarction (14).

References

Tkachenko, E. et al. (2005) Circ. Res. 96:488.
Reizes, O. et al. (2008) Int. J. Biochem. Cell Biol. 40:28.
Carey, D.J. et al. (1997) J. Biol. Chem. 272:2873.
ENTREZ protein Accession # O75056, EAX076736 and EAX07637.
Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
Schultz, J.G. et al. (2003) J. Biol. Chem. 278:48651.
Hienola, A. et al. (2006) J. Cell Biol. 174:569.
Goutebroze, L. et al. (2003) BMC Neurosci. 4:29.
Kaksonen, M. et al. (2002) Mol. Cell. Neurosci. 21:158.
Strader. A.D. et al. (2004) J. Clin. Invest. 114:1354.
Cornelison, D.D.W. et al. (2004) Genes Dev. 18:2231.
Fisher, M.C. et al. (2006) Matrix Biol. 25:27.
Pacifici, M. et al. (2005) J. Bone Miner. Metab. 23:191.
Finsen, A.V. et al. (2004) Physiol. Genomics 16:301.

Entrez Gene IDs

9672 (Human); 20970 (Mouse)

Alternate Names

KIAA0468; N-Syndecan; SDC3; SDCN; SYND3syndecan proteoglycan 3; syndecan 3 (N-syndecan); syndecan 3; syndecan neural type; Syndecan3; Syndecan-3

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Citations for Human Syndecan-3 Isoform 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer
Authors: LL Lin, ER Kost, CL Lin, P Valente, CM Wang, MG Kolonin, AC Daquinag, X Tan, N Lucio, CN Hung, CP Wang, NB Kirma, TH Huang
Cell Rep, 2020-10-13;33(2):108253.
Species: Human
Sample Types: Whole Cells
Applications: CyTof
Contribution of syndecans to cellular uptake and fibrillation of alpha-synuclein and tau
Authors: A Hudák, E Kusz, I Domonkos, K Jósvay, AT Kodamullil, L Szilák, M Hofmann-Ap, T Letoha
Sci Rep, 2019-11-12;9(1):16543.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Co-Immunoprecipitation
Detection of Glycan Shedding in the Blood: New Class of Multiple Sclerosis Biomarkers?
Authors: Brian DellaValle, Alba Manresa-Arraut, Henrik Hasseldam, Allan Stensballe, Jørgen Rungby, Agnete Larsen et al.
Frontiers in Immunology
Attachment and Post-Attachment Receptors Important for Hepatitis C Virus Infection and Cell-to-Cell Transmission
Authors: H Fan, L Qiao, KD Kang, J Fan, W Wei, G Luo
J. Virol., 2017-06-09;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot