Human Syndecan-4 APC-conjugated Antibody Summary
Ser20-Glu145
Accession # P31431
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Syndecan‑4 in Activated Lymphocytes in Human PBMCs by Flow Cytometry. Activated lymphocytes in human peripheral blood mononuclear cells were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Rat Anti-Human Syndecan-4 APC-conjugated Monoclonal Antibody (Catalog # FAB29181A) or (B) Rat IgG2AAllophycocyanin Isotype Control (IC006A). View our protocol for Staining Membrane-associated Proteins.
View Larger
Detection of Syndecan‑4 in U87MG Human Cell Line by Flow Cytometry. U87MG human glioblastoma cell line was stained with Rat Anti-Human Syndecan-4 APC-conjugated Monoclonal Antibody (Catalog # FAB29181A, filled histogram) or Rat IgG2A Allophycocyanin Isotype Control (IC006A,open histogram). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
Background: Syndecan-4
Syndecan-4, previously known as Amphiglycan or Ryudocan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying Heparan Sulfate (HS) and Chondroitin Sulfate (CS) glycosaminoglycans. The four vertebrate syndecans have two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. Syndecan-4 is the most similar to Syndecan-2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGF-beta 2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator (1, 2). Human Syndecan-4 is synthesized as a 198 amino acid (aa) core protein with an 18 aa signal sequence, a 127 aa extracellular domain containing three consensus Ser-Gly sequences for the attachment of HS side chains, a 25 aa transmembrane region and a 28 aa cytoplasmic tail (3). Human Syndecan-4 ECD shares approximately 79%, 78% and 81% aa sequence identity with mouse, rat and porcine Syndecan-4 ECD, respectively. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 20 kDa core protein. Non-covalent homodimerization of Syndecan-4 is dependent on the transmembrane domain (4). The HS chains can bind fibronectin, SDF-1, antithrombin, FGF-2, midkine and tissue factor pathway inhibitor and can present FGF‑2 to its receptors (1, 2, 5). Proteolytic cleavage by plasmin, thrombin or a metalloproteinase may create a functional ectodomain (6‑8). Genetic disruption of the Syndecan-4 gene causes a mild phenotype, presumably due to compensation by other syndecans, but mice have an increase in placental thrombi as well as defects in wound healing and response to endotoxin shock (9, 10).
- Tkachenko, E. et al. (2005) Circ. Res. 96:488.
- Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
- David, G. et al. (1992) J. Cell Biol. 118:961.
- Choi, S. et al. (2005) J. Biol. Chem. 280:42573.
- Charnaux, N. et al. (2005) FEBS J. 272:1937.
- Schmidt, A. et al., J. Biol. Chem. 280:34441.
- Rauch, B. H. et al. (2005) J. Biol. Chem. 280:17507.
- Fitzgerald, M. L. et al. (2000) J. Cell Biol. 148:811.
- Ishiguro, K. et al. (2003) Glycoconj. J. 19:315.
- Echtermeyer, F. et al. (2001) J. Clin. Invest. 107:R9.
Product Datasheets
Citations for Human Syndecan-4 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
3
Citations: Showing 1 - 3
Filter your results:
Filter by:
-
Biodistribution and Cellular Internalization of Inactivated SARS-CoV-2 in Wild-Type Mice
Authors: A Hudák, G Morgan, J Bacovsky, R Patai, TF Polgár, A Letoha, A Pettko-Sza, C Vizler, L Szilák, T Letoha
International Journal of Molecular Sciences, 2022-07-09;23(14):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Contribution of Syndecans to the Cellular Entry of SARS-CoV-2
Authors: A Hudák, A Letoha, L Szilák, T Letoha
International Journal of Molecular Sciences, 2021-05-19;22(10):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Contribution of syndecans to cellular internalization and fibrillation of amyloid-?(1-42)
Authors: T Letoha, A Hudák, E Kusz, A Pettkó-Sza, I Domonkos, K Jósvay, M Hofmann-Ap, L Szilák
Sci Rep, 2019-02-04;9(1):1393.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Syndecan-4 APC-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human Syndecan-4 APC-conjugated Antibody and earn rewards!
Have you used Human Syndecan-4 APC-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
