Human Syntabulin Antibody

Catalog #: AF5544 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5544
AF5544-SP

Detection of Human Syntabulin by Western Blot.

1 Image

All Syntabulin Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Syntabulin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Syntabulin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Syntabulin
Gln489-Arg656
Accession # Q9NX95

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human Syntabulin by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Syntabulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5544) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Syntabulin at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syntabulin

Syntabulin (also Golgi-localized synaphilin-related protein) is a 70-75 kDa molecule that belongs to the syntaxin-binding and kinesin-1 family of motor adaptor proteins. It is highly spliced, with various forms associated with different tissues. In neurons, it anchors syntaxin-1 to KIF5B, creating a complex by which synaptic components can be transported to synaptic terminals. Human Syntabulin is a type II transmembrane (TM) protein 663 amino acids (aa) in length. It contains one coiled-coil region (aa 271‑353) and a TM domain between aa 606‑626. Alternate splice forms show alternate start sites at Met120 and Met207, a five aa substitution for aa 1‑8, a 16 aa insertion after Lys8, and an eight aa substitution for aa 1‑76. Over aa 486‑653, human Syntabulin shares 83% aa identity with mouse Syntabulin.

Entrez Gene IDs

55638 (Human); 319613 (Mouse); 500865 (Rat)

Alternate Names

Golgi-Localized Protein; Golgi-Localized Syntaphilin-Related Protein; GOLSYN A Protein; GOLSYN B Protein; GOLSYN C Protein; GOLSYN; Implicated In Syntaxin Trafficking In Neurons; KIAA1472; Microtubule-Associated Protein; OCSYN; SNPHL; SYBU; Syntabulin (Syntaxin-Interacting); Syntabulin; syntaphilin-like; Syntaxin-1-Binding Protein