Human TACC3 Antibody Summary
Glu689-Ile838
Accession # Q9Y6A5
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TACC3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TACC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5720) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TACC3 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Detection of Human TACC3 by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for TACC3 at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TACC3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5720) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TACC3
Transforming acidic coiled-coil containing protein 3 (TACC3; also know as ERIC-1) is a 92‑140 kDa member of the TACC family of transcriptional regulatory proteins. It is expressed in a variety of cell types, including Sertoli cells, endothelial cells, erythroid progenitors, and thyroid epithelium. When phosphorylated by Aurora A on Ser558, it stabilizes the mitotic spindle. It also interacts with nuclear histone acetyltransferases, promoting their positive effect on transcription. Human TACC3 is 838 amino acids (aa) in length and contains one poly-Serine region (aa 155‑160) and a TACC coiled-coil domain (aa 637‑837). There is one potential splice variant that shows a Lys substitution for aa 102‑462. Over amino acids 689‑838, human TACC3 shares 79% aa identity with mouse TACC3.
Product Datasheets
Citations for Human TACC3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
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Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cells.
Authors: Ana G, Bernardo O, Marco N et al.
Curr Biol.
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A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.
Authors: So C, Seres K. B, et al.
Science
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Polo-like kinase 1 promotes Cdc42-induced actin polymerization for asymmetric division in oocytes
Authors: WS Yuen, QH Zhang, A Bourdais, D Adhikari, G Halet, J Carroll
Open Biology, 2023-03-08;13(3):220326.
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Targeting FGFRs by pemigatinib induces G1 phase cell cycle arrest, cellular stress and upregulation of tumor suppressor microRNAs
Authors: Pace, A;Scirocchi, F;Napoletano, C;Zizzari, IG;Po, A;Megiorni, F;Asquino, A;Pontecorvi, P;Rahimi, H;Marchese, C;Ferretti, E;Nuti, M;Rughetti, A;
Journal of translational medicine
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cells.
Authors: Ana G, Bernardo O, Marco N et al.
Curr Biol.
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Defining endogenous TACC3–chTOG–clathrin–GTSE1 interactions at the mitotic spindle using induced relocalization
Authors: Ellis L. Ryan, James Shelford, Teresa Massam-Wu, Richard Bayliss, Stephen J. Royle
Journal of Cell Science
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FGFR3-TACC3 fusion proteins act as naturally occurring drivers of tumor resistance by functionally substituting for EGFR/ERK signaling
Authors: C Daly, C Castanaro, W Zhang, Q Zhang, Y Wei, M Ni et al.
Oncogene
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