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Human TAFA4/FAM19A4 Antibody

Catalog #: MAB5099 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5099
MAB5099-SP
Detection of TAFA4/FAM19A4 in A172 Human Cell Line by Flow Cytometry.
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Human TAFA4/FAM19A4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TAFA4/FAM19A4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human TAFA1, 2, 3, or 5 is observed.
Source
Monoclonal Mouse IgG1 Clone # 480103
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TAFA4
Ser35-Arg140
Accession # Q96LR4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TAFA4/FAM19A4 (Catalog # 5099-TA) under non-reducing conditions only
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of TAFA4/FAM19A4 antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TAFA4/FAM19A4 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human TAFA4/FAM19A4 Monoclonal Antibody (Catalog # MAB5099, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAFA4/FAM19A4

TAFA4 (also FAM19A4) is a secreted, 12 kDa member of the FAM19/TAFA family of chemokine-like proteins (1). It is synthesized as a 140 amino acid (aa) precursor that contains a 35 aa signal sequence and a 105 aa mature chain. Like other members of the FAM19/TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues that follow the pattern Cx7CCx13CxCx14Cx11Cx4Cx5Cx10C, where C represents a conserved cysteine residue and x represents any noncysteine amino acid (1). Human TAFA4 is 90% aa identical to mouse TAFA4 (1). Real-time PCR analysis indicates that TAFA4 mRNA expression is restricted to the central nervous system (CNS), with the highest level in the thalamus (1). TAFAs may modulate immune responses in the CNS by functioning as brain specific chemokines and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1, 2). TAFAs may also control axonal sprouting following brain injury (1).

References
  1. Tang, Y.T. et al. (2004) Genomics 83:727.
  2. Benveniste, E. (1998) Cytokine Growth Factor Rev. 9:259.
Long Name
Family with Sequence Similarity 19, Member A4
Entrez Gene IDs
151647 (Human); 320701 (Mouse)
Alternate Names
Chemokine-like protein TAFA-4; FAM19A4; family with sequence similarity 19 (chemokine (C-C motif)-like), member A4; FLJ25161; TAFA4; TAFA-4

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