Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all TCF7L1/TCF3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TCF7L1
Lys429-Ser581
Accession # Q9HCS4

Specificity

Detects human TCF7L1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human TCF7L1 Antibody

Detection of Human TCF7L1 antibody by Western Blot.

Detection of Human TCF7L1 by Western Blot.

Western blot shows lysates of human pancreas and human lung tissue. PVDF Membrane was probed with 0.2 µg/mL of Human TCF7L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6116) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TCF7L1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TCF7L1 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

TCF7L1 in BG01V Human Stem Cells.

TCF7L1 was detected in immersion fixed BG01V human embryonic stem cells using Human TCF7L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, upper panel; Catalog # NL012) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human TCF7L1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Western Blot

0.2 µg/mL
Sample: Human pancreas and human lung tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TCF7L1/TCF3

Transcription factor 7-like 1 (TCF7L1; also HMG box transcription factor 3, TCF-3) is a 63 kDa member of the TCF/LEF family of proteins. Human TCF7L1 is 588 amino acids (aa) in length and contains one HMG box DNA-binding domain (aa 346-414). Human TCF7L1 shares 95% aa sequence identity with mouse TCF7L1. TCF7L1 is expressed in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium. Functionally, it participates in the Wnt signaling pathway. TCF7L1 binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. TCF7L1 is also necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis.

Long Name

Transcription Factor 7-like 1

Alternate Names

TCF3

Entrez Gene IDs

83439 (Human); 21415 (Mouse); 312451 (Rat)

Gene Symbol

TCF7L1

UniProt

Q9HCS4 (Human)

Additional TCF7L1/TCF3 Products

Product Documents for Human TCF7L1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TCF7L1 Antibody

For research use only

Citations for Human TCF7L1 Antibody

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Protocols

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