Human TDAG8/GPR65 Antibody

Catalog #: MAB10077 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10077-100
MAB10077-SP

Detection of Human TDAG8/GPR65 by Western Blot.

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All TDAG8/GPR65 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TDAG8/GPR65 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TDAG8/GPR65 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 976128

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human TDAG8/GPR65
Met1-Glu337
Accession # Q81IYL9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TDAG8/GPR65 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human TDAG8/GPR65. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TDAG8/GPR65 Monoclonal Antibody (Catalog # MAB10077) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for TDAG8/GPR65 at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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TDAG8/GPR65 in Transfected HEK293 Human Cell Line. TDAG8/GPR65 was detected in immersion fixed TDAG8/GPR65-transfected HEK293 human embryonic kidney cell line (left panel, positive stain) and non-transfected HEK293 human embryonic kidney cell line (right panel, negative stain) using Mouse Anti-Human TDAG8/GPR65 Monoclonal Antibody (Catalog # MAB10077) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TDAG8/GPR65

Human G Protein-Coupled Receptor 65 (GPR65), also known as T-Cell Death-Associated Gene 8 Protein (TDAG8) and Psychosine Receptor, is a 337 aminoacids proton-sensing GPCR primarily expressed in lymphoid tissues and is uniquely expressed in Th17 cells; mice deficient in GPR65 are resistant to autoimmunity. GPR65 senses pH by protonation of histidine residues on its extracellular domain. Many pH-sensing G protein-coupled receptors have emerged as potential therapeutic agents in conditions that involve the response to acidotic stress.

Long Name

G Protein-coupled Receptor 65

Entrez Gene IDs

8477 (Human); 14744 (Mouse); 299242 (Rat)

Alternate Names

G protein-coupled receptor 65; GPR65; G-protein coupled receptor 65; Psychosine Receptor; T-cell death-associated gene 8 protein; TDAG8; TDAG8hTDAG8