Human TERT Antibody
Human TERT Antibody Summary
Glu281-Ala436
Accession # O14746
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TERT by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human TERT Monoclonal Antibody (Catalog # MAB6595) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TERT at approximately 127 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human TERT by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for TERT at approximately 155 kDa (as indicated) using 40 µg/mL of Mouse Anti-Human TERT Monoclonal Antibody (Catalog # MAB6595). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TERT
Telomerase reverse transcriptase (TERT) is a 127 kDa member of the reverse transcriptase family of ribonucleoprotein enzymes that are essential for replication of chromosome termini. Human TERT is 1132 amino acids (aa) in length and contains one reverse transcriptase domain (aa 605-935). Splicing variants produce three isoforms for human TERT. Isoform 1 is the long form. Isoform 2 has a 44 aa substitution for aa 764-807 in isoform 1, and a deletion of aa 808-1132. Isoform 3 has a deletion of aa 885-947. Human TERT shares 62% aa sequence identity with mouse and rat TERT. Defects in TERT are associated with a susceptibility to aplastic anemia, are a cause of dyskeratosis congenital autosomal dominant, and increase susceptibility to idiopathic pulmonary fibrosis.
Product Datasheets
Citation for Human TERT Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Constitutive activation of STAT3 signaling regulates hTERT and promotes stem cell-like traits in human breast cancer cells.
Authors: Chung S, Aroh C, Vadgama J
PLoS ONE, 2013-12-30;8(12):e83971.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
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