Home / TFCP2L1 / Human TFCP2L1 Antibody

Human TFCP2L1 Antibody

Catalog #: AF5726
19 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5726
AF5726-SP
Detection of Human TFCP2L1 by Western Blot.
1 Image
Product Details
Citations (19)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TFCP2L1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TFCP2L1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TFCP2L1
Met1-Lys180
Accession # Q9NZI6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TFCP2L1 antibody by Western Blot. View Larger

Detection of Human TFCP2L1 by Western Blot. Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TFCP2L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5726) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TFCP2L1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFCP2L1

TFCP2L1 (Transcription factor CP2‑like protein # 1; also LBP‑9) is a 54 kDa (predicted) member of the CP2 subfamily, grh/CP2 family of proteins. It is expressed in placental synctiotrophoblast cells, and regulates the production of CYP11A1/P450scc. P450scc is a cholesterol cleavage enzyme that generates pregnenolone, a necessary intermediate in the formation of progesterone. TFCP2L1 both positively and negatively regulates P450scc synthesis through homodimerization and heterodimerization with LBP‑1b. Human TFCP2L1 is 479 amino acids (aa) in length. It contains a transcriptional repressor region (aa 100‑200) and a dimerization domain (aa 300‑479). There is one splice variant that shows a deletion of aa 400‑464. Human TFCP2L1 is 73% aa identical to human TFCP2, and 93% aa identical to mouse CRTR‑1, a closely related molecule that may not represent the actual mouse ortholog to TFCP2L1.

Long Name
Transcription factor CP2-like 1
Entrez Gene IDs
29842 (Human); 81879 (Mouse); 304741 (Rat)
Alternate Names
CRTR1; CRTR1CRTR-1; LBP9; LBP-9; LBP9CP2-related transcriptional repressor 1; TFCP2L1; transcription factor CP2-like 1; transcription factor CP2-like protein 1; Transcription factor LBP-9

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human TFCP2L1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Resetting Transcription Factor Control Circuitry toward Ground-State Pluripotency in Human.
    Authors: Takashima Y, Guo G, Loos R et al.
    Cell
  2. Spatial Transcriptional Mapping of the Human Nephrogenic Program
    Authors: Lindstrom NO, Sealfon R, Chen X et al.
    Dev Cell
  3. Principles of signaling pathway modulation for enhancing human naive pluripotency induction
    Authors: J Bayerl, M Ayyash, T Shani, YS Manor, O Gafni, R Massarwa, Y Kalma, A Aguilera-C, M Zerbib, H Amir, D Sheban, S Geula, N Mor, L Weinberger, S Naveh Tass, V Krupalnik, B Oldak, N Livnat, S Tarazi, S Tawil, E Wildschutz, S Ashouokhi, L Lasman, V Rotter, S Hanna, D Ben-Yosef, N Novershter, S Viukov, JH Hanna
    Cell Stem Cell, 2021-04-28;0(0):.
  4. Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States
    Authors: AJ Collier, SP Panula, JP Schell, P Chovanec, A Plaza Reye, S Petropoulo, AE Corcoran, R Walker, I Douagi, F Lanner, PJ Rugg-Gunn
    Cell Stem Cell, 2017-03-23;0(0):.
  5. Bat pluripotent stem cells reveal unusual entanglement between host and viruses
    Authors: Marion Déjosez, Arturo Marin, Graham M. Hughes, Ariadna E. Morales, Carlos Godoy-Parejo, Jonathan L. Gray et al.
    Cell
  6. Revealing cell populations catching the early stages of human embryo development in naive pluripotent stem cell cultures
    Authors: M Moya-Jódar, A Ullate-Ago, P Barlabé, JR Rodríguez-, G Abizanda, C Barreda, X Carvajal-V, A Vilas-Zorn, JP Romero, L Garate, X Agirre, G Coppiello, F Prósper, XL Aranguren
    Stem Cell Reports, 2022-12-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Differentiation of human induced pluripotent stem cells into hypothalamic vasopressin neurons with minimal exogenous signals and partial conversion to the naive state
    Authors: H Ozaki, H Suga, M Sakakibara, M Soen, N Miyake, T Miwata, S Taga, T Nagai, M Kano, K Mitsumoto, T Miyata, T Kobayashi, M Sugiyama, T Onoue, H Takagi, D Hagiwara, S Iwama, R Banno, G Iguchi, Y Takahashi, K Muguruma, H Inoue, H Arima
    Scientific Reports, 2022-10-17;12(1):17381.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  8. The transcription factor TFCP2L1 promotes primordial germ cell-like cell specification of pluripotent stem cells
    Authors: M Zhang, J Ji, X Wang, X Zhang, Y Zhang, Y Li, X Wang, X Li, Q Ban, SD Ye
    The Journal of Biological Chemistry, 2021-09-21;0(0):101217.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  9. Histone Acetyltransferase MOF Blocks Acquisition of Quiescence in Ground-State ESCs through Activating Fatty Acid Oxidation
    Authors: LTP Khoa, YC Tsan, F Mao, DM Kremer, P Sajjakulnu, L Zhang, B Zhou, X Tong, NV Bhanu, C Choudhary, BA Garcia, L Yin, GD Smith, TL Saunders, SL Bielas, CA Lyssiotis, Y Dou
    Cell Stem Cell, 2020-06-30;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  10. Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells
    Authors: K Takahashi, D Jeong, S Wang, M Narita, X Jin, M Iwasaki, SD Perli, BR Conklin, S Yamanaka
    Cell Rep, 2020-06-02;31(9):107715.
    Species: Human
    Sample Types:
    Applications: ICC
  11. Identification of ALPPL2 as a Naive Pluripotent State-Specific Surface Protein Essential for Human Naive Pluripotency Regulation
    Authors: Y Bi, Z Tu, Y Zhang, P Yang, M Guo, X Zhu, C Zhao, J Zhou, H Wang, Y Wang, S Gao
    Cell Rep, 2020-03-17;30(11):3917-3931.e5.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, ICC, Western Blot
  12. Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency
    Authors: N Bredenkamp, J Yang, J Clarke, GG Stirparo, F von Meyenn, S Dietmann, D Baker, R Drummond, Y Ren, D Li, C Wu, M Rostovskay, S Eminli-Mei, A Smith, G Guo
    Stem Cell Reports, 2019-11-07;13(6):1083-1098.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Distinct Molecular Trajectories Converge to Induce Naive Pluripotency
    Authors: Hannah T. Stuart, Giuliano G. Stirparo, Tim Lohoff, Lawrence E. Bates, Masaki Kinoshita, Chee Y. Lim et al.
    Cell Stem Cell
  14. Honey bee Royalactin unlocks conserved pluripotency pathway in mammals
    Authors: DC Wan, SL Morgan, AL Spencley, N Mariano, EY Chang, G Shankar, Y Luo, TH Li, D Huh, SK Huynh, JM Garcia, CM Dovey, J Lumb, L Liu, KV Brown, A Bermudez, R Luong, H Zeng, VL Mascetti, SJ Pitteri, J Wang, H Tu, M Quarta, V Sebastiano, R Nusse, TA Rando, JE Carette, JF Bazan, KC Wang
    Nat Commun, 2018-12-04;9(1):5078.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys
    Authors: Y Kang, Z Ai, K Duan, C Si, Y Wang, Y Zheng, J He, Y Yin, S Zhao, B Niu, X Zhu, L Liu, L Xiang, L Zhang, Y Niu, W Ji, T Li
    Cell Rep, 2018-11-27;25(9):2563-2576.e9.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Whole Cells
    Applications: ICC
  16. Transcription Factors Drive Tet2-Mediated Enhancer Demethylation to Reprogram Cell Fate
    Authors: JL Sardina, S Collombet, TV Tian, A Gómez, B Di Stefano, C Berenguer, J Brumbaugh, R Stadhouder, C Segura-Mor, M Gut, IG Gut, S Heath, S Aranda, L Di Croce, K Hochedling, D Thieffry, T Graf
    Cell Stem Cell, 2018-09-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunoprecipitation
  17. Trim33 regulates early maturation of mouse embryoid bodies in vitro
    Authors: S Rajderkar, C Panaretos, V Kaartinen
    Biochem Biophys Rep, 2017-10-18;12(0):185-192.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  18. alpha-Intercalated cells defend the urinary system from bacterial infection.
    Authors: Paragas N, Kulkarni R, Werth M, Schmidt-Ott K, Forster C, Deng R, Zhang Q, Singer E, Klose A, Shen T, Francis K, Ray S, Vijayakumar S, Seward S, Bovino M, Xu K, Takabe Y, Amaral F, Mohan S, Wax R, Corbin K, Sanna-Cherchi S, Mori K, Johnson L, Nickolas T, D'Agati V, Lin C, Qiu A, Al-Awqati Q, Ratner A, Barasch J
    J Clin Invest, 2014-06-17;124(7):2963-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  19. Single-Cell Profiling Reveals Sex, Lineage, and Regional Diversity in the Mouse Kidney.
    Authors: Ransick, A, LindstrOm, N O Et al.
    Dev Cell

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human TFCP2L1 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human TFCP2L1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human TFCP2L1 Antibody
By Anonymous on 05/31/2018
Application: WB Sample Tested: Human embryonic stem cells (H9 from Wicell) Species: Human

1:500 concentration used