Human TFF2 Antibody

Name: Human TFF2 Antibody
Brand: R&D Systems
SKU: MAB4077
Rating: 5 (1 reviews)

Catalog #: MAB4077
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4077
MAB4077-SP

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All TFF2 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TFF2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TFF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human TFF3.

Source

Monoclonal Mouse IgG_2B Clone # 366508

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TFF2
Glu24-Tyr129
Accession # Q03403

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human TFF2

Immunohistochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TFF2 in Human Stomach. TFF2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human TFF2 Monoclonal Antibody (Catalog # MAB4077) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to gastric glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFF2

Trefoil Factor 2 (TFF2), previously called spasmolysin, is one of three trefoil peptides secreted by epithelial cells that line mucus membranes. Trefoils contribute to protection and repair of the gastrointestinal tract. TFF2 is secreted by epithelia of the basal gastric glands and duodenal Brunner’s glands, but is also found in spleen, thymus and lung and may modulate leukocyte recruitment to sites of inflammation in these tissues. The 129 amino acid (aa) human TFF2 contains two trefoil structures formed by intramolecular disulfides and shows 82% aa identity with mouse TFF2.

Long Name

Trefoil Factor 2

Entrez Gene IDs

7032 (Human); 21785 (Mouse); 116592 (Rat)

Alternate Names

SML1; SML1trefoil factor 2, SML1, human spasmolytic polypeptide (SP)10spasmolytic protein 1; SP; Spasmolysin; Spasmolytic Polypeptide; TFF2; trefoil factor 2

Product Datasheets

Product Datasheet

COA

SDS

Citation for Human TFF2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Nardilysin regulates inflammation, metaplasia, and tumors in murine stomach
Authors: Y Kimura, K Ikuta, T Kimura, T Chiba, H Oshima, M Oshima, E Nishi, H Seno
Sci Rep, 2017-02-23;7(0):43052.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC